- 询价
- Cell Signaling Technology
- 5013
- USA
- 2025年09月08日
- W, IP, F
- H,M,R,Mk,Mi,Dm,Z,B,Pg
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Biotinylated)
- 抗原：
synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase
- 应用范围：
W, IP, F
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
H,M,R,Mk,Mi,Dm,Z,B,Pg
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Mi=Mink Dm=D. melanogaster Z=Zebrafish B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP F | H M R Mk Mi Dm Z B Pg | Endogenous | 42, 44 | Rabbit IgG |
| Specificity / Sensitivity | p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Biotinylated) detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. The antibody does not cross-react with JNK/SAPK or p38 MAP kinase. |
|---|---|
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase. Western Blotting
Western blot analysis of extracts from various cell lines using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Biotinylated). Flow Cytometry
Flow cytometric analysis of Jurkat cells, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Biotinylated) (blue) compared to XP® Rabbit (DA1E) mAb IgG Isotype Control (Biotinylated) #4096 (red). |
| Description | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The unconjugated p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 reacts with human, mouse, rat, monkey, mink, pig, Saccharomyces cerevisiae, Drosophila melanogaster, hamster, bovine and zebrafish p44/42 MAPK protein. CST expects that p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Biotinylated) will also recognize MAPK in these species. |
| Background | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit monoclonal antibody is produced Produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
, YWHAZ. Cell Cycle Proteins Regulated by the Erk1/2 Pathway: CCNA1, CCNA2, CCNB1, CCNB2, CCND1, CCND2, CCND3, CCNE1, CCNE2, CDK2, CDK4, CDK6, CDKN1A, CDKN1B, CDKN1C, CDKN2A, CDKN2B, CDKN2C, CDKN2D, E2F1, RB1. 2。T细胞与B细胞活化基因芯片 T细胞与B细胞活化基因芯片列有适应性免疫
. IPKA HA-tagged MAPK (a variation & alternate protocol for MAPK IPKA) 1) Transfect cells with GFP and pcDNAI-ERK1-HA plasmids 2) Transfected cells were allowed to adhere to fibronectin-coated dishes for the given time and then stimulated
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