- 询价
- Cell Signaling Technology
- 4807
- USA
- 2025年07月15日
- W, IP, F
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Stat5a (4H1) Mouse mAb
- 抗原：
recombinant fragment expressing amino acids 2-104 of human Stat5a
- 应用范围：
W, IP, F
- 保质期：
详见说明书
- 适应物种：
H
- 级别：
详见MSDS文件
- 库存：
大量
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP F | H | Endogenous | 90 | Mouse IgG2b |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Stat5a (4H1) Mouse mAb detects endogenous levels of total human Stat5a protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a recombinant fragment expressing amino acids 2-104 of human Stat5a. Western Blotting
Western blot analyis of extracts from K-562, MOLT-4 and TF-1 cells using Stat5a (4H1) Mouse mAb. Flow Cytometry
Flow cytometric analysis of HeLa cells (red) and K-562 cells (blue) using Stat5a (4H1) Mouse mAb. |
| Background | Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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