Phospho-p44/42 MAPK (Erk1/2) (

点击跳转到CST中国官网了解详情

http://www.cellsignal.com/iproducts/4370.html

Applications	Dilution	Species-Reactivity	Sensitivity	MW (kDa)	Isotype
W	1:2000	Human,Mouse,Rat,Hamster,Monkey,Mink,D. melanogaster,Zebrafish,Bovine,Dog,Pig,S. cerevisiae	Endogenous	44, 42	Rabbit IgG
IP	1:50
IHC-P	1:400
F	1:800
IF-IC	1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry)

Homology

Species predicted to react based on 100% sequence homology: Chicken, C. elegans

Specificity / Sensitivity

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases.

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb兔单抗能够检测内源性的Thr202和Tyr204被双重磷酸化的Erk1(Thr185和 Tyr187双重磷酸化的Erk2)，以及Thr202被单独磷酸化的p44和p42 MAPK(Erk1和Erk2)蛋白。该抗体与JNK/SAPK或p38 MAPK的相应磷酸化残基没有交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase.

该单克隆抗体是采用合成的与人源p44 MAPK的Thr202/Tyr204位点周围残基一致的磷酸化肽段免疫动物而产生的。

Background

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.

丝裂原活化蛋白激酶(MAPKs)是一个具有广泛保守性的丝氨酸/苏氨酸蛋白激酶家族，在许多细胞过程中起作用，比如细胞增殖、分化、运动和死亡。p44/42 MAPK (Erk1/2)信号转导通路能在应答各种胞外刺激后被激活，这些刺激因子包括有丝分裂原、生长因子和细胞因子(1-3)，并且被研究者认为是诊断和治疗癌症的重要靶点(4)。受到刺激后，三个不同层次的蛋白激酶级联反应就连续被开启，这三个层次的分子包含MAPKKK（MAP3K）、MAPKK（MAP2K）、MAPK。多种p44/42 MAP3Ks已经被鉴定出来，包括Raf家族成员，以及Mos、Tpl2/Cot。MEK1、MEK2是该通路中主要的MAPKKs(5,6)。MEK1、MEK2通过分别磷酸化p44和p42蛋白的活化环内的Thr202/Tyr204位点和Thr185位点/Tyr187位点，从而激活p44、p42。目前已经鉴定出p44/42的一些下游靶点，包括p90RSK (7) 和下游转录因子Elk-1 (8,9)。双特异性(Thr/Tyr) MAPK磷酸酶家族成员DUSPs、MKPs (10)，以及MEK抑制子如U0126、 PD98059，可以负调节p44/42。

1. Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
2. Baccarini, M. (2005) FEBS Lett 579, 3271-7.
3. Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
4. Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
5. Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
6. Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
7. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
8. Marais, R. et al. (1993) Cell 73, 381-93.
9. Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
10. Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.

Application References

• Zhao, K.W. et al. (2015) J Cell Biochem 116, 767-77.Applications:
• Lai, C.Y. et al. (2014) Stem Cells , .Applications:Flow Cytometry
• Hutchins, E.J. and Szaro, B.G. (2013) J Neurosci 33, 14666-80.Applications:Western Blotting
• Faber, A. et al. (2011) Cancer Discov 1, 352-365.Applications:IHC-P (paraffin),Western Blotting
• Kippenberger, S. et al. (2010) Biochim Biophys Acta 1803, 940-950.Applications:IF-IC (In Cells),Western Blotting
• Rohani, M.G. et al. (2010) BMC Immunol 11, 53.Applications:Western Blotting
• Benedettini, E. et al. (2010) Am J Pathol 177, 415-23.Applications:Western Blotting
• Moritz, A. et al. (2010) Sci Signal 3, ra64.Applications:Western Blotting
• Hudon, V. et al. (2010) J Med Genet 47, 182-9.Applications:IHC-P (paraffin)
• Mabilleau, G. and Sabokbar, A. (2009) PLoS ONE 4, e4173.Applications:Western Blotting
• Koenigsmann, J. et al. (2009) Blood 113, 4690-701.Applications:Flow Cytometry
• Schrage, Y.M. et al. (2009) Cancer Res 69, 6216-22.Applications:Western Blotting
• Miyaji, M. et al. (2009) Proc Natl Acad Sci U S A 106, 14502-7.Applications:Western Blotting
• Kubo, T. et al. (2009) J Exp Med 206, 1971-82.Applications:Western Blotting
• Gray, M.J. et al. (2008) J Natl Cancer Inst 100, 109-20.Applications:Western Blotting
• Rygiel, T.P. et al. (2008) J Cell Sci 121, 1183-92.Applications:Western Blotting
• Engelman, J.A. et al. (2008) Nat Med 14, 1351-6.Applications:IHC-P (paraffin),Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

4370 :

Western Blotting Immunoprecipitation (Agarose) Immunohistochemistry (Paraffin) Flow Immunofluorescence*

Companion Products

• 1150 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Blocking Peptide
• 12630 SignalFire™ Plus ECL Reagent
• 12757 SignalFire™ Elite ECL Reagent
• 4376 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb
• 4377 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb
• 4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
• 4696 p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb
• 5425 Normal Goat Serum
• 6883 SignalFire™ ECL Reagent
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7727 Biotinylated Protein Ladder Detection Pack
• 8059 SignalStain^® DAB Substrate Kit
• 8103 SignalSlide^® Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Controls
• 8112 SignalStain^® Antibody Diluent
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)
• 8201 PhosphoPlus^® p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody Duet
• 9101 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody
• 9102 p44/42 MAPK (Erk1/2) Antibody
• 9106 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb
• 9194 p44/42 MAPK (Erk1/2) Control Cell Extracts
• 9997 Tris Buffered Saline with Tween^® 20 (TBST-10X)