- 询价
- Cell Signaling Technology
- 4286
- USA
- 2025年12月15日
- W, IP
- H,M
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Oct-4 (9B7) Mouse mAb
- 抗原：
recombinant human Oct-4 protein
- 应用范围：
W, IP
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 适应物种：
H,M
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M | Endogenous | 45 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Oct-4 (9B7) Mouse mAb detects endogenous levels of total Oct-4 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with recombinant human Oct-4 protein. Western Blotting
Western blot analysis of extracts from NTERA and F9 cell lines using Oct-4 (9B7) Mouse mAb. |
| Background | Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Phenotyping of Mouse Models with OCT
Optical coherence tomography (OCT) is an invaluable technique to perform noninvasive retinal imaging in small animal models such as mice. It provides virtual cross sections that correlate well with histomorphometric data with the advantage
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
) to the Protein A column. Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10 the amount of antibody compared with other subclasses
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