- 询价
- Cell Signaling Technology
- 9186
- USA
- 2025年07月15日
- W
- H,M,R
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-Elk-1 (Ser383) (2B1) Mouse mAb
- 抗原：
synthetic phosphopeptide corresponding to residues around Ser383 of human Elk-1
- 应用范围：
W
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
H,M,R
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | (H) (M) (R) | Recombinant | 47 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Elk-1 (Ser383) (2B1) Mouse mAb detects less than 5 ng of phosphorylated Elk-1, and will not react with up to 1 µg of nonphosphorylated Elk-1 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser383 of human Elk-1. Western Blotting
Western blot analysis of Elk-1 fusion protein expressed from E. coli with or without phosphorylation with purified Erk2 enzyme, using Phospho-Elk-1 (Ser383) (2B1) Mouse mAb (upper) or Elk-1 Antibody #9182 (lower). |
| Background | The transcription factor Elk-1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors (1-3). Elk-1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its carboxy terminus; phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk-1. Elk-1 appears to be a direct target of activated MAP kinase: (a) biochemical studies indicate that Elk-1 is a good substrate for MAP kinase; (b) the kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity; (c) interfering mutants of MAP kinase block Elk-1 activation in vivo. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK (4,5). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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