- 询价
- Cell Signaling Technology
- 3929
- USA
- 2026年05月28日
- W, F
- H,M,R,Mk,Dm
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- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated)
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1
- 应用范围：
W, F
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk,Dm
- 库存：
大量
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W F | H M R Mk Dm | Endogenous | 15 to 20 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at analagous sites. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1. Western Blotting
Western blot analysis of extracts from HeLa cells, serum-starved and stimulated with insulin (100 nM for 5 minutes), with (+) or without (-) λ phosphatase treatment, using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) (upper) and 4E-BP1 (53H11) Rabbit mAb #9644 (lower). Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002, Wortmannin and U0126-treated (blue), using Phospho-4E-BP1 (Thr36/46) (236B4) Rabbit mAb (Biotinylated). |
| Description | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855. |
| Background | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
IF/mIHC/空间组学切片选型指南：选对冰冻、FFPE少走弯路
9101 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody 一抗 4060 Phospho-Akt (Ser473) (D9E) Rabbit Monoclonal Antibody 一抗 S0B2194 S-RMab® CD163 Recombinant Rabbit mAb (SDT-R164) 一抗 S0B2103 alpha smooth muscle Actin (SMA) Recombinant Rabbit mAb
2S5I) Rabbit mAb 抗体 41255S SLK Antibody 抗体 9062S Phospho-PLK1 (Thr210) (D5H7) Rabbit mAb 抗体 S0B0035 PLK1 Recombinant Rabbit mAb (SDT-R062) 抗体 abs580421 α-酮戊二酸单钠盐 小分子化合物 四、苯乙酰化（Phenylacetylation） 2026年6月，中国科学院上海药物研究所谭敏佳/李静雅/徐骏宇团队与华东理工大学叶邦策团队合作，在《Cell
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