- 询价
- Cell Signaling Technology
- 3513
- USA
- 2026年05月28日
- IP
- All
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- 文献和实验
- 技术资料
- 抗体英文名：
GST (26H1) Mouse mAb (Sepharose Bead Conjugate)
- 抗原：
GST fusion protein
- 应用范围：
IP
- 适应物种：
All
- 级别：
详见MSDS文件
- 库存：
大量
- 供应商：
CST
- 保质期：
详见说明书
- 标记物：
Sepharose Bead
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
400 ul (40 immunoprecipitations)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 400 ul (40 immunoprecipitations) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IP=Immunoprecipitation
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IP | All | Transfected Only | Mouse IgG2a |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | GST (26H1) Mouse mAb (Sepharose Bead Conjugate) detects transfected GST fusion proteins. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a GST fusion protein. IP
Immunoprecipitation of extracts from COS cells transfected with a construct overexpressing TrCp1-GST using GST (26H1) Mouse mAb (Sepharose Bead Conjugate) (lane 2). Mouse IgG Isotype Control (Sepharose Bead Conjugate) #3420 (lane 1) was used as a negative control. The western blot was probed using GST (91G1) Rabbit mAb #2625. |
| Description | This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. GST (26H1) Mouse mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Purification of GST Fused Proteins
mM DTT/1%TX-100 and place on wheel for 30 min. Wash 2X in PBS/10mM DTT/1%TX-100. Alternatively, use pre-swollen Pharmacia Glutathione Sepharose beads. These can be washed rapidly 3 times in PBS/10mM DTT/1%TX100. Pellet debris 20 min
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
contains the fusion protein. Run the samples from the eluates containing protein on an SDS-polyacrylamide gel and stain with Coomassie blue dye. The GST moiety is 26 kD; therefore, add 26 kD to the predicted molecular weight of your fusion protein
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
4.5 (without removing prehybridization solution.) C. Repeat the addition of the 2XSSC wash twice more. Remove the mix and wash twice, 30 min each time, in 2XSSC pH7/0.1% CHAPS 70 °C. Wash twice, 10 min each, in Maleic Acid Buffer ( MAB; 100 mM maleic acid
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