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- 英文名:
SureBeads™ Trial Kit Protein A #1614811
- 库存:
2
- 供应商:
研卉生物
- 规格:
rack
Trial kit for protein A conjugated magnetic beads for immunoprecipitation, contains 1 ml bead suspension (10 mg beads/ml suspension) and a 4-tube magnetic rack
Use this trial kit (available for a limited time) for small-scale immunoprecipitation experiments to evaluate SureBeads Protein A Magnetic Beads.
Kit contents:
- 1 ml vial of protein A conjugated magnetic beads; 10 mg (10 mg beads/ml)
- 4-tube magnetic rack
SureBeads Protein A Magnetic Beads enable fast, easy, and reliable bioseparations: immunoprecipitation (IP), co-immunoprecipitation (co-IP), and protein pull-down assays.
Superparamagnetic beads with a surface-activated hydrophilic polymer and chemically conjugated protein A bind the Fc regions of immunoglobulins with high specificity.
- Faster IP using magnetization instead of centrifugation
- Unique bead surface chemistry ensures optimal antibody orientation for increased antigen binding efficiency
- High IgG binding affinity with low nonspecific binding and reduced antibody consumption
- Beads cling magnetically to the side wall of the tube, not the bottom, for easier wash pipetting
Packaging Options
| Conjugated Protein | Bead Suspension Volume | Rack Size (Number of Tubes) |
Catalog # |
| G | 1 ml | — | 161-4021 |
| G | 3 ml | — | 161-4023 |
| G | 1 ml | 4* | 161-4821 |
| A | 1 ml | — | 161-4011 |
| A | 3 ml | — | 161-4013 |
| A | 1 ml | 4* | 161-4811 |
| A/G | 2 x 3 ml | 16 | 161-4833 |
| G/G | 2 x 3 ml | 16 | 161-4823 |
| A/A | 2 x 3 ml | 16 | 161-4813 |
| 16-tube magnetic rack | 16 | 161-4916 | |
*Trial kit
Related Products
More Information
Protein A and protein G have different binding affinities for various species and subclasses of monoclonal and polyclonal antibodies. Use the table below to select the best SureBeads products for your needs.
Protein A/G Immunoprecipitation Guide
| Monoclonal | Protein A | Protein G |
| Human IgG1 | +++ | +++ |
| Human IgG2 | +++ | +++ |
| Human IgG3 | – | +++ |
| Human IgG4 | +++ | +++ |
| Mouse IgG1 | + | +++ |
| Mouse IgG2a | +++ | +++ |
| Mouse IgG2b | ++ | ++ |
| Mouse IgG3 | ++ | ++ |
| Rat IgG1 | – | + |
| Rat IgG2aa | – | +++ |
| Rat IgG2b | – | ++ |
| Rat IgG2c | + | ++ |
| Polyclonal | Protein A | Protein G |
| Rabbit | +++ | ++ |
| Cow | ++ | +++ |
| Horse | ++ | +++ |
| Goat | – | ++ |
| Guinea pig | +++ | ++ |
| Sheep | +/- | ++ |
| Pig | ++ | ++ |
| Rat | +/- | ++ |
| Mouse | ++ | ++ |
| Chicken | – | + |
| Human IgG | +++ | +++ |
| Human IgM | – | + |
| Human IgD | – | + |
| Human IgA | – | + |
– No binding
+ Weak binding
++ Moderate binding
+++ Strong binding
| 1614013 | SureBeads? Protein A Magnetic Beads, 3 ml | SureBeads磁珠Protein A |
| 1614023 | SureBeads? Protein G Magnetic Beads, 3 ml | SureBeads磁珠Protein G |
| 1614811 | SureBeads? Trial Kit Protein A | SureBeads磁珠Protein A加磁力架 |
| 1614821 | SureBeads™ Trial Kit Protein G | SureBeads磁珠Protein G加磁力架 |
| 1614813 | SureBeads™ Immunoprecipitation Starter Kit A/A | SureBeads磁珠 加磁力架 |
| 1614823 | SureBeads™ Immunoprecipitation Starter Kit G/G | SureBeads磁珠 加磁力架 |
| 1614833 | SureBeads™ Immunoprecipitation Starter Kit A/G | SureBeads磁珠 加磁力架 |
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个以上,其它作为三级。 5.漂洗:把李坯在清水中漂洗去盐,至略带咸味时为止。再将其晾晒至七成干后,去核。 6.配料:李坯100公斤,砂糖50公斤,甘草10公斤,茴香800克,桂尔通1公斤,桔皮油200克,糖精50克,安息香酸钠40克,柠檬酸适量。 7.吸糖:先将甘草、茴香煎成浓汁,加适量糖精、安息香酸钠、配制成60%的浓糖液。将糖液倒入李坯,根据不同口味,加适量柠檬酸。待全部李坯吸足糖液,即可入锅烧煮。 8.烧煮:将李坯连同糖液倒入锅内,加热煮沸,煮至果肉熟透而不软
是 Fc 区域,这个 Fc 区域就能结合 Protein A 或 G。自从磁珠出现之后,CoIP 的操作就变得更加简单了,只需要用包被 Protein A 或 G 的磁珠先和抗体结合,然后用抗体磁珠复合体去结合蛋白 B,之后再用 PAGE 胶分离,用 WB 检测就 OK 了。除了研究蛋白质相互作用,同样的原理,还可以用来研究核转录因子,有了这样的实验,必定为文章增色不少。那么知道了原理,我们可以先在网上找几个视频看看,从视频中我们知道了 CoIP 的原理,以及做 CoIP 都需要什么,那么我们就动手
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