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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2; NP_001243728.1.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX89409
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Goat
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody is expected to recognize both reported isoforms (NP_002037.2; NP_001243728.1).
- 抗体英文名:
GAPDH antibody, Internal
- 抗体名:
GAPDH 抗体, Internal
- 规格:
100 μg
WB analysis of HeLa (A) and NIH-3T3 (B) cell lysate using GTX89409 GAPDH antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer
ICC/IF analysis of PFA fixed U251 cells using GTX89409 GAPDH antibody, Internal.
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
IHC-P analysis of human liver using GTX89409 GAPDH antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml
WB analysis of human liver (A), testes (B) and tonsil (C) lysate using GTX89409 GAPDH antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
WB analysis of mouse liver (A) and rat heart (B) lysate using GTX89409 GAPDH antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
ICC/IF analysis of PFA fixed HeLa cells using GTX89409 GAPDH antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 5μg/ml
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文献和实验GAPDH抗体|GAPDH抗体大全-艾美捷指导您购买合适的GAPDH抗体
,但是进口品牌如Abcam、Millipore、CST等价格偏高,一般在¥3000/100ul左右。下面我们为您推荐三款GAPDH原装进口抗体,分别是美国EarthOx和ProteinTech的产品。EarthOx的Anti-GAPDH Monoclonal Antibody (货号E021010 )是小鼠源的单抗,其效价很高,厂商推荐的WB起始稀释比率为1:1000,实际应用过程中,有使用1:10000或更高的报告,远远高于此稀释比率,说明其免疫效价很高,另外,其检测种属也很多,包括人、牛、猪、小鼠
has become the method of choice for routine viral diagnosis. As false-negative test results can have serious implications; an internal/external control system should be incorporated in each analysis to detect RT-qPCR failure due to poor sample quality, improper nucleic
Use of Internal and External Standards or Reference RNAs for
under investigation are compared from sample to sample using an internal control to normalize for differences in sample concentration and loading. Ideally, the internal control should be a gene expressed at a constant level across the sample set. In absolute
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