- ￥5900
- GeneTex
- 美国
- GTX12288
- 2025年07月13日
- WB, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide corresponding to a sequence at the C-terminus of human Hamartin(1150-1164aa QLHIMDYNETHHEHS), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX12288
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
500 μg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Hamartin / TSC1
- 抗体英文名：
Hamartin / TSC1 antibody
- 抗体名：
Hamartin / TSC1 抗体
- 规格：
100 μg
IHC-P analysis of rat brain tissue using GTX12288 Hamartin / TSC1 antibody.
WB analysis of various samples using GTX12288 Hamartin / TSC1 antibody.
Lane 1 : rat skeletal muscle tissue lysate at 50ug
Lane 2 : rat heart tissue lysate at 50ug
Lane 3 : rat brain tissue lysate at 50ug
Lane 4 : rat lung tissue lysate at 50ug
Lane 5 : 293T whole cell lysate at 40ug
Lane 6 : HeLa whole cell lysate at 40ug
Lane 7 : HT1080 whole cell lysate at 40ug
Lane 8 : SMMC whole cell lysate at 40ug
Dilution : 0.5 μg/mL
IHC-P analysis of human mammary cancer tissue using GTX12288 Hamartin / TSC1 antibody.
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
. The TSC1 gene contains 23 exons with 21 exons coding for a 8.6 kb transcript which is translated into the protein hamartin. The TSC2 gene product tuberin is encoded by an mRNA of 5.5 kb consisting of 41 exons.
There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
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