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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of human EXPAND1 / MUM1 expressed in E. Coli.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX82832
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, IHC-P, ELISA
- 靶点:
EXPAND1 / MUM1
- 抗体英文名:
EXPAND1 / MUM1 antibody [4G10]
- 抗体名:
EXPAND1 / MUM1 抗体 [4G10]
- 规格:
100 μl
IHC-P analysis of human Uterus tissue using GTX82832 EXPAND1 / MUM1 antibody [4G10].
IHC-P analysis of human melanoma (A), non-Hodgkin`s lymphoma (B) using GTX82832 EXPAND1 / MUM1 antibody [4G10].
WB analysis of human EXPAND1 / MUM1 (AA: 590-711) recombinant protein using GTX82832 EXPAND1 / MUM1 antibody [4G10].
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文献和实验to a tube containing 10 ml of spleen cell media (1 x 104 dilution). For very thickly growing cells, make two dilutions into washing media (1 x 106 dilution). Plate 36 wells of a 96 well tissue culture plate with 0.2 ml of this mixture (7.2
Protocol for Limiting Dilutions
that appear to be monoclonal for antibody production (ELISA). Freeze strong positive wells (as given below), and expand the cell line in vitro using a large tissue culture flask or in vivo by initiation of ascites fluid in mice (BALB/c).
Kamps's Western Blotting Protocol
in immunoprecipitates than anti-immunoglobulin antibodies.ECL analysis of immunoprecipitates using a second antibody is almost always blighted by strong staining of the antibody bands and by the staining of abundant proteins in the sample.Blots developed with iodinated
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