- ￥5600
- GeneTex
- 美国
- GTX81198
- 2025年07月19日
- WB, IHC-P, FACS
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH conjugated synthetic peptide between 172-201 amino acids from the N-terminal region of human TBC1D4.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX81198
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P, FACS
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
AS160 / TBC1D4
- 抗体英文名：
AS160 / TBC1D4 antibody, N-term
- 抗体名：
AS160 / TBC1D4 抗体, N-term
- 规格：
400 μl
FACS analysis of MDA-MB231 cells using GTX81198 AS160 / TBC1D4 antibody, N-term.
Top histogram : negative control
Bottom histogram : MDA-MB231 cells
WB analysis of MDA-MB231 cell lysate (35ug/lane) using GTX81198 AS160 / TBC1D4 antibody, N-term.
IHC-P analysis of human skeletal muscle using GTX81198 AS160 / TBC1D4 antibody, N-term.
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
标本。 2. 加20ul 1 N HC I ,盖紧，上下混匀。 2 － 8 ℃ 放置60± 2 分钟。 3. 加20ul 1 N NaOH, 盖紧，上下混匀。 4. 即用，或放-20/-70 ℃ 保存3 天。计算结果时乘 以稀释倍数 50 。 （ 注意：不同的标本E2F1 的水平可能有较大差异，请根据实际情况灵活掌握稀释度） 5. 细胞培养上清或组织匀浆 10 倍稀释 (410ul 的标本
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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