- ￥5300
- GeneTex
- 美国
- GTX79230
- 2025年07月10日
- WB, IHC-Fr, FACS, IP, Activation, IHC
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human TCR V alpha 12.1
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX79230
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, IHC-Fr, FACS, IP, Activation, IHC
- 浓度：
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
TCR V alpha 12.1
- 抗体英文名：
TCR V alpha 12.1 antibody [6D6.6]
- 抗体名：
TCR V alpha 12.1 抗体 [6D6.6]
- 规格：
200 μg
FACS analysis of PBMC cells using GTX79230 TCR V alpha 12.1 antibody [6D6.6] compared to an isotype control (blue).
Dilution : 2 ug/test incubated for 30 min at 4ºC
WB analysis of 25 ug of Jurkat (lane 1), CEM (lane 2) and rat thymus (lane 3) cell lysates using GTX79230 TCR V alpha 12.1 antibody [6D6.6].
Dilution : 1:10
FACS analysis of positive PEER cells (left panel) or negative control Jurkat cells (right panel) using GTX79230 TCR V alpha 12.1 antibody [6D6.6].
Dilution : 5ul of primary antibody were used per test
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文献和实验网络 二、TCR基因的重排 T细胞在胸腺中发育成熟过程中，TCR基因按照一定的顺序发生重排。TCR基因的重排顺序和表达与免疫球蛋白基因的重排和表达十分相似。在 基因组 中的识别序列包括了一个保守的七聚体和九聚体，七聚体与九聚体之间含有一个不保守的12碱基对或23碱基对间隔序列（spacer sequence
In situ hybridisation to alpha satellite sequences (chromosome specific)
anti-mouse HRP antibody diluted 1:80 for 40 minutes at 37C We use DAKO P0260 (12) Wash with blocking solution for 10 minutes (13) Incubate with swine anti-rabbit HRP antibody diluted 1:100 for 40 minutes at 37C We use DAKO P0217 (14
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
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