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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX70163
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IP
- 浓度:
1.86 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
E2F1
- 抗体英文名:
E2F1 antibody [16G7]
- 抗体名:
E2F1 抗体 [16G7]
- 规格:
100 μl
E2F1 antibody [16G7] detects E2F1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: E2F1 protein stained by E2F1 antibody [16G7] (GTX70163) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [16G7] (GTX70163) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.
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文献和实验Chen HL et al., Anticancer Res 2020 (PMID:32878783)
Zhong WB et al., Int J Mol Sci 2018 (PMID:29932104)
Wang LT et al., Cancer Lett 2021 (PMID:34265398)
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上,标准品和样品中的 E2F1与单抗结合,加入生物素化的抗人 E2F1 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值,E2F1 浓度与 OD 值成正比
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