- ￥1700 - 4000
- GeneTex
- 美国
- GTX633388
- 2025年07月09日
- WB, ICC/IF, IHC-P
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys5 of human Histone H2A. The exact sequence is proprietary.
- 亚型：
IgG3
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX633388
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
0.96 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H2AK5ac (acetyl Lys5)
- 抗体英文名：
Histone H2AK5ac (acetyl Lys5) antibody [GT1262]
- 抗体名：
Histone H2AK5ac (acetyl Lys5) 抗体 [GT1262]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] detects Histone H2AK5ac (acetyl Lys5) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H2AK5ac (acetyl Lys5) protein stained by Histone H2AK5ac (acetyl Lys5) antibody [GT1262] (GTX633388) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102078) diluted at 1:300.
Scale bar = 10 μm.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2AK5ac (acetyl Lys5) antibody [GT1262] (GTX633388) diluted at 1:1000.
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] detects Histone H2AK5ac (acetyl Lys5) protein at nucleus in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] (GTX633388) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] detects Histone H2AK5ac (acetyl Lys5) protein at nucleus in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] (GTX633388) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Huang FI et al., Front Oncol 2019 (PMID:31024851)
，为此CST公司推出了可用于蛋白功能检测的乙酰化组蛋白系列抗体,圆满的解决这个问题。因为乙酰化与蛋白的活性相关，因此通过乙酰化的系列抗体特异性地识别靶蛋白的乙酰化形式，可以反映出靶蛋白质的活性水平，达到研究蛋白功能和调节的目的。而其他的抗体仅能反映出该蛋白质的表达量或表达水平，不能反映出蛋白的活性状态。乙酰化系列抗体：Catalog#Product DescriptionSourceApplications2576Acetyl-Histone H2A (Lys5) AntibodyRabbitW IP
from the antibody-bound and antibody-unbound fractions (from Chromatin Immunoprecipitation on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications). Control genomic DNA should be used as well. For each PCR reaction, we use 20-50 ng of template
Identifying Acetylated Proteins in Mitosis
the acetylation status of several mitotic proteins by anti-acetyl-Lysine immunoprecipitation with a monoclonal antibody followed by Western blot analyses of the proteins of interest. We further confirmed by a reciprocal immunoprecipitation with protein-specific
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