The immunogen used to generate this antibody corresponds to SARS virus nsp8.
亚型:
IgG1
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
SARS Coronavirus, SARS Coronavirus 2
保质期:
12 months from the shipping date of the product.
抗原来源:
SARS Coronavirus
目录编号:
GTX632696
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Mouse
应用范围:
WB, ICC/IF, IHC-Fr, FACS
浓度:
0.67 mg/ml (Please refer to the vial label for the specific concentration.)
Non-infected (–) and infected (+, 48h pl MOI 0.01) whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:5000.
SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) detects SARS-CoV-2 (COVID-19) NSP8 by flow cytometry analysis. Sample: Vero E6 cells infected with SARS-CoV-2. Black: Uninfected Vero E6 cells was used as a control. Red: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) dilution: 1:100.
SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] detects SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% PFA at RT for 15 min.Green: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 stained by SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
Application Reference
Kotaki T et al., Sci Rep 2021 (PMID:33500537)
Cell Death Dis. 2020;11(8)
Hirano M et al., Nat Biotechnol 2022 (PMID:35468954)
Patrick Madden et al., Res Sq 2022 (PMID:35411346)
Madden PJ et al., bioRxiv 2022 (PMID:35262081)
Ter Ellen BM et al., Viruses 2021 (PMID:34372541)
Ciccosanti F et al., Antiviral Res 2021 (PMID:33781803)