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beta Tubulin 3/ Tuj1 antibody

[GT11710]
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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • GTX631836
  • 2025年07月15日
  • WB, ICC/IF, IHC-P, IHC-Fr, IP
  • Mouse
  • Human, Mouse, Rat, Fish
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 3/ Tuj1. The exact sequence is proprietary.

    • 亚型

      IgG2b

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse, Rat, Fish

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX631836

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Mouse

    • 应用范围

      WB, ICC/IF, IHC-P, IHC-Fr, IP

    • 浓度

      1 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      beta III Tubulin antibody [GT11710] specifically recognizes endogenous levels of beta III Tubulin based on the immunogen sequence.

    • 抗体英文名

      beta Tubulin 3/ Tuj1 antibody [GT11710]

    • 抗体名

      beta Tubulin 3/ Tuj1 抗体 [GT11710]

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein expression by immunofluorescent analysis.
    Sample: Cultured rat E18 primary hippocampal neuron. Cells were fixed in 4% PFA at RT for 15 min.
    Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
    Blue: Fluoroshield with DAPI (GTX30920).

    产品细节图片2

    beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
    Sample: Frozen sectioned adult mouse retina.
    Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
    Blue: Fluoroshield with DAPI (GTX30920).

    产品细节图片3

    beta III Tubulin antibody [GT11710] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis.
    Sample:Frozen section of embryonic mouse brain (mE18.5).
    Red: beta III Tubulin antibody [GT11710] (GTX631836) diluted at 1:500.
    Blue: DAPI

    产品细节图片4

    beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
    Sample: Frozen sectioned E13.5 rat brain.
    Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250.
    Blue: Fluoroshield with DAPI (GTX30920).

    产品细节图片5

    beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
    Sample: Frozen sectioned E13.5 rat brain.
    Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
    Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250.
    Blue: Fluoroshield with DAPI (GTX30920).

    产品细节图片6

    Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

    产品细节图片7

    Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT11710] (GTX631836).
    Western blot analysis was performed using beta III Tubulin antibody [GT11710] (GTX631836).
    EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

    产品细节图片8

    beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

    产品细节图片9

    Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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    图标文献和实验
    该产品被引用文献
    Application Reference
    Zhao Y et al., Neurogastroenterol Motil 2020 (PMID:32515097)
    Hsu WH et al., Aging (Albany NY) 2020 (PMID:32501291)
    Guallar D et al., Cell Stem Cell 2020 (PMID:32396862)
    Ruiz-Palacios M et al., Sci Total Environ 2020 (PMID:31791770)
    Lee WJ et al., Cancer Med 2019 (PMID:31274246)
    Tomoyuki Kurose et al., Stem Cell Research 2019 (Epub)
    Kikuchi J et al., Biochem Biophys Res Commun 2019 (PMID:31409482)
    Chen YH et al., Sci Rep 2019 (PMID:31289326)
    Borgo C et al., Sci Rep 2017 (PMID:28209983)
    Liu SP et al., Biomedicine (Taipei) 2015 (PMID:25705583)
    Jin C et al., Bioeng Transl Med 2023 (PMID:36925694)
    Ma PW et al., Oxid Med Cell Longev 2022 (PMID:36531206)
    Hosoya M et al., Dev Neurobiol 2021 (PMID:34545999)
    Makoto Hosoya et al., Neural Dev 2022 (PMID:35524278)
    Jia-Wei Chen et al., Front Cell Neurosci 2022 (PMID:35210991)
    Jennifer Yejean Kim et al., Front Neurosci 2022 (PMID:35401074)
    Yulou Yu et al., Front Cell Neurosci 2021 (PMID:34220452)
    Simone Keck et al., Cell Mol Gastroenterol Hepatol 2021 (PMID:33741501)
    Yifeng Yang et al., Commun Biol 2022 (PMID:35379867)
    Vajh?j C et al., PLoS One 2021 (PMID:34932569)
    Hosoya M et al., FEBS J 2021 (PMID:32323465)
    Rey F et al., Aging Cell 2021 (PMID:34799977)
    Chang KH et al., Stem Cell Res Ther 2021 (PMID:34551822)
    Otsuka T et al., Stem Cells Dev 2021 (PMID:34148410)
    Chen C et al., Front Cell Dev Biol 2021 (PMID:33912567)
    Shinjyo N et al., J Ethnopharmacol 2021 (PMID:33129946)
    Carelli S et al., Nanotheranostics 2021 (PMID:33391972)
    Ruiz-Palacios M et al., Fish Shellfish Immunol 2020 (PMID:32771610)
    相关实验
    • Immunofluorescent Localization of Tubulin

      antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film

    • Immunofluorescent Localization of Tubulin

      Rabbit anti-tubulin (or other primary antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter

    • Antibody cleanup

      for this purpose.   Grow cells in 200 mls of YPD at 30oC to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7. Fix for 1 hour at room temperature with shaking. Centrifuge cells at 3000 X g. Resuspend

    图标技术资料

    暂无技术资料 索取技术资料

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    文献支持
    beta Tubulin 3/ Tuj1 antibody [GT11710]
    ¥1700 - 4000