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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys9 of human Histone H3. The exact sequence is proprietary.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX630554
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Histone H3K9ac (Acetyl Lys9)
- 抗体英文名:
Histone H3K9ac (Acetyl Lys9) antibody [GT464]
- 抗体名:
Histone H3K9ac (Acetyl Lys9) 抗体 [GT464]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Histone H3K9ac (acetyl Lys9) antibody [GT464] detects Histone H3K9ac (acetyl Lys9) protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Tricostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H3K9ac (acetyl Lys9) antibody [GT464] (GTX630554) dilution: 1:5000
Histone H3K9ac (acetyl Lys9) antibody [GT464] detects Histone H3K9ac (acetyl Lys9) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H3K9ac (acetyl Lys9) protein stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] (GTX630554) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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文献和实验Hsia CR et al., iScience 2022 (PMID:36117991)
) AntibodyRabbitW IHC IC9671Acetyl-Histone H3 (Lys9) AntibodyRabbitW IP IHC IC2591Acetyl-Histone H4 (Lys12) AntibodyRabbitW IP IHC IC2594Acetyl-Histone H4 (Lys8) AntibodyRabbitW IP IHC IC有时组蛋白同时乙酰化和磷酸化修饰,进行转录的调控和活性的调节,因此CST也推出了能同时检测乙酰化和磷酸化的组蛋白的抗体。该系列抗体只识别同时乙酰化和磷酸
Identifying Acetylated Proteins in Mitosis
the acetylation status of several mitotic proteins by anti-acetyl-Lysine immunoprecipitation with a monoclonal antibody followed by Western blot analyses of the proteins of interest. We further confirmed by a reciprocal immunoprecipitation with protein-specific
from the antibody-bound and antibody-unbound fractions (from Chromatin Immunoprecipitation on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications). Control genomic DNA should be used as well. For each PCR reaction, we use 20-50 ng of template
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