- ￥1700 - 4000
- GeneTex
- 美国
- GTX630545
- 2025年07月09日
- WB, ICC/IF, IP, Dot, ChIP assay
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding di-methyl Lys20 of human Histone H4. The exact sequence is proprietary.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX630545
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IP, Dot, ChIP assay
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H4K20me2 (dimethyl Lys20)
- 抗体英文名：
Histone H4K20me2 (dimethyl Lys20) antibody [GT1851]
- 抗体名：
Histone H4K20me2 (dimethyl Lys20) 抗体 [GT1851]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Dot blot analysis of various of H4K20 methylation peptides using Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (GTX630545) at antibody dilution of 1:500.
Un : H4 protein peptide
Me : H4K20 mono-methyl peptide
2Me : H4K20 di-methyl peptide
3Me : H4K20 Tri-methyl peptide
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.
Histone H4 (di methyl Lys20) antibody [GT1851] detects Histone H4 (di methyl Lys20) protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
Histone H4 (di methyl Lys20) antibody [GT1851] (GTX630545) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Immunoprecipitation of HIST1H4A protein from HeLa whole cell extracts using 5 μg of Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (GTX630545).
Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (GTX630545).
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
Histone H4 (di-Methyl Lys20) antibody [GT1851] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT1851] (GTX630545) diluted at 1:500.
Blue: Hoechst 33342 staining.
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文献和实验Wang Y et al., PLoS One 2021 (PMID:34185806)
Ye S et al., Cancer Res 2019 (PMID:31308046)
Paquin KL et al., Mol Cell Biol 2019 (PMID:31085681)
Dai L et al., Mol Cell Biol 2016 (PMID:28031330)
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
dishes of lysed E. coli to reduce anti-E. coli antibody background. These antibodies are present in all human sera, rabbit sera, and rodent sera. For each Petri dish containing solidified LB agar, mix 0.6ml of the Y1090 overnight-saturated culture with 30
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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