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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue virus NS3 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Dengue virus
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Dengue virus 2
- 目录编号:
GTX629477
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Dengue virus NS3 protein
- 抗体英文名:
Dengue virus NS3 protein antibody [GT2811]
- 抗体名:
Dengue virus NS3 protein 抗体 [GT2811]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody [GT2811] (GTX629477) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein by western blot analysis.
A. 66 μg BHK-21 whole cell extract
B. 20 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 10 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
10% SDS-PAGE
NS3 (Dengue virus) antibody [GT2811] (GTX629477) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in PFA.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody [GT2811] (GTX629477) diluted at 1:2000.
Blue: Hoechst 33342 staining.
NS3 (Dengue Virus 2) antibody [GT2811] detects NS3 protein by western blot analysis.
A. 30 μg BHK21 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells
C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
7.5% SDS-PAGE
NS3 (Dengue Virus 2) antibody [GT2811] (GTX629477) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in PFA.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody [GT2811] (GTX629477) diluted at 1:2000.
Blue: Hoechst 33342 staining.
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文献和实验Cerikan B et al., Cell Rep 2020 (PMID:32640225)
Ngo AM et al., Elife 2019 (PMID:31516121)
Frakolaki E et al., Cells 2019 (PMID:31387309)
Cortese M et al., Cell Rep 2019 (PMID:31141684)
Tohma D et al., Arch Virol 2018 (PMID:30357482)
Liu X et al., Virology 2018 (PMID:30172996)
Liu H et al., J Virol 2017 (PMID:28228593)
Lai JH et al., EMBO Rep 2018 (PMID:29880709)
Brugier A et al., J Virol 2022 (PMID:35266803)
Sarah Goellner et al., STAR Protoc 2020 (PMID:33377067)
Jenn-Haung Lai et al., iScience 2021 (PMID:34142025)
Wesley Freppel et al., Bio Protoc 2018 (PMID:34532557)
Mazeaud C et al., Viruses 2021 (PMID:34696522)
Labeau A et al., J Virol 2020 (PMID:31915280)
against dengue, timely diagnosis is therefore necessary for patient management. Laboratory diagnosis is carried out by virus isolation, demonstration of viral antigen, presence of viral nucleic acid, and antibodies. Further, recombinant dengue envelope protein
Fluorimetric and HPLC-Based Dengue Virus Protease Assays Using a FRET Substrate
The number of dengue virus infections is increasing and the dengue NS2B–NS3 protease is considered a promising target for the development of antiviral therapies. Therefore, reliable and fast screening systems are needed for the discovery
Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris
The full-length and truncated forms of recombinant envelope (E) glycoprotein from Dengue virus type 1, Singapore strain S275/90 were expressed in the yeast, Pichia pastoris , using a secretory vector. A truncated form of the E protein
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