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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
HRP
- 适应物种:
Human, Mouse, Rat, Zebrafish
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX628802-01
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB
- 浓度:
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
alpha Tubulin
- 抗体英文名:
alpha Tubulin antibody [GT114] (HRP)
- 抗体名:
alpha Tubulin 抗体 [GT114] (HRP)
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (HRP) (GTX628802-01) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (HRP) (GTX628802-01) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (HRP) (GTX628802-01) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Tubulin antibody [GT114] (HRP) detects alpha Tubulin protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
alpha Tubulin antibody [GT114] (HRP) (GTX628802-01) dilution: 1:10000
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文献和实验Waller V et al., Front Oncol 2023 (PMID:36998455)
Monta??s-Agudo P et al., J Cell Sci 2021 (PMID:34859816)
In situ hybridisation to alpha satellite sequences (chromosome specific)
anti-mouse HRP antibody diluted 1:80 for 40 minutes at 37C We use DAKO P0260 (12) Wash with blocking solution for 10 minutes (13) Incubate with swine anti-rabbit HRP antibody diluted 1:100 for 40 minutes at 37C We use DAKO P0217 (14
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
immunofluorescence. Block in AbDil for 10'. Anti-tubulin for 20' - 30' (We use DM1alpha) Wash 4x TBST (TBST = TBS + 0.1% Triton X-100) Secondary for 20' - 30' Wash 4X TBST. Wash once with TBST + 1 ug/ml Hoechst
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
Biology International''31(6):638-644. 货号3011,产品描述:Goat anti-Rabbit IgG Secondary antibody unconjugated; 货号3012,产品描述:Goat anti-Rabbit IgG Secondary antibody HRP conjugated; 货号3021,产品描述:Goat anti-Human IgG
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