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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
HRP
- 适应物种:
Goat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Goat
- 目录编号:
GTX628481-01
- 级别:
Secondary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB
- 浓度:
0.22 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Mouse Anti-Goat IgG (Light chain)
- 抗体英文名:
Mouse Anti-Goat IgG (Light chain) antibody [GT25612] (HRP)
- 抗体名:
Mouse Anti-Goat IgG (Light chain) 抗体 [GT25612] (HRP)
- 规格:
250 μl
Mouse anti-Goat light chain antibody
Comparison of western blot analysis with mouse anti-Goat light chain antibody (GTX628481-01 diluted at 1:5000) (1) and traditional secondary antibody (GTX228416-01 diluted at 1:5000) (2).
A. 2 μg denature goat IgG
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文献和实验IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
human antibodies, we use Sigma #A-3150: goat anti-human IgG alkaline phosphatase conjugate, diluted 1:1000) Wash membranes in TBST for 5 minutes, 4 times at room temperature. Develop membranes by diluting 33 µl NBT and 66µl BCIP in 10
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
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