- ￥1700 - 4000
- GeneTex
- 美国
- GTX628480
- 2025年05月19日
- WB, ICC/IF, IHC-P, IP
- Mouse
- Human, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The immunogen used to generate this antibody corresponds to human HIF1 alpha
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX628480
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
HIF1 alpha
- 抗体英文名：
HIF1 alpha antibody [GT10211]
- 抗体名：
HIF1 alpha 抗体 [GT10211]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody [GT10211] (GTX628480) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
HIF1 alpha antibody [GT10211] detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: HIF1 alpha stained by HIF1 alpha antibody [GT10211] (GTX628480) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
HIF1 alpha antibody [GT10211] detects HIF1A protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract (untreated)
B. 30 μg HepG2 whole cell lysate/extract (1%O2 treatment for 48hr)
7.5% SDS-PAGE
HIF1 alpha antibody [GT10211] (GTX628480) dilution: 1:250
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
HIF-1 alpha antibody immunoprecipitates HIF-1 alpha protein in IP experiments.
IP Sample: 1000 μg HepG2 whole cell lysate/extract
A. 40 μg HepG2 whole cell lysate/extract from normoxia
B. 40 μg HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2)
C. Control with 2.5 μg of preimmune mouse IgG/HepG2 whole cell
lysate/extract from normoxia
D. Control with 2.5 μg of preimmune mouse IgG/ HepG2 whole cell
lysate/extract from hypoxia 24hr (1%O2)
E. Immunoprecipitation of HIF-1 alpha protein by 2.5 μg of HIF-1
alpha antibody (GTX628480)/HepG2 whole cell lysate/extract
from normoxia
F. Immunoprecipitation of HIF-1 alpha protein by 2.5 μg of HIF-1
alpha antibody (GTX628480)/HepG2 whole cell lysate/extract
from hypoxia 24hr (1%O2)
10% SDS-PAGE
The immunoprecipitated HIF-1 alpha protein was detected by HIF-1 alpha antibody (GTX628480) diluted at 1:500.
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
HIF1 alpha antibody detects HIF1 alpha protein at nuclear by confocal immunofluorescent analysis. Sample: Hypoxia (500μM CoCl2) treated 24hr (right) or untreated (left) HeLa cells were fixed in 4% PFA for 15 min. Red: HIF1 alpha protein stained by HIF1 alpha antibody (GTX628480) diluted at 1:250. Green: Alpha-tubulin, a cytoskeleton marker, stained by Rabbit Polyclonal antibody (GTX102078) diluted at 1:500. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
HIF1 alpha antibody [GT10211] detects HIF1 alpha protein by Western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract (untreated)
B. 30 μg HepG2 whole cell lysate/extract (500 μM CoCl2 treatment for 24 hr)
7.5 % SDS-PAGE
HIF1 alpha antibody [GT10211] (GTX628480) dilution: 1:500
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文献和实验Keitoku M et al., J Clin Med 2022 (PMID:36555975)
Yao-Lung Chang et al., Genes (Basel) 2022 (PMID:35627137)
Chen Zhang et al., Int J Med Sci 2021 (PMID:34522167)
Beak JY et al., J Biol Chem 2021 (PMID:34756888)
Sharma S et al., Hum Mol Genet 2017 (PMID:28204537)
Saki M et al., Mol Cancer Res 2017 (PMID:28801308)
Sharma S et al., Genome Biol 2019 (PMID:30791937)
Cho SY et al., Evid Based Complement Alternat Med 2013 (PMID:23589723)
Masola V et al., Oncotarget 2018 (PMID:30546836)
Shih JW et al., Nat Commun 2017 (PMID:28639619)
Matsumura-Matsuda E et al., Intern Med 2018 (PMID:29269645)
Berezowska S et al., Virchows Arch 2017 (PMID:28062918)
Bourseau-Guilmain E et al., Nat Commun 2016 (PMID:27094744)
Biao Ma et al., Nat Cell Biol. 2014 (PMID:25438054)
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
dishes of lysed E. coli to reduce anti-E. coli antibody background. These antibodies are present in all human sera, rabbit sera, and rodent sera. For each Petri dish containing solidified LB agar, mix 0.6ml of the Y1090 overnight-saturated culture with 30
Antibody Screening of Bacteriophage gt11 DNA Expression Libraries
Much of our current understanding of the molecular details of the activity and interactions of proteins has stemmed from the ability to isolate cDNA encoding these proteins. Computer-based analysis of deduced amino acid sequence allows
Antibody Screening of Bacteriophage gt-11 DNA Expression Libraries
Much of our current understanding of the molecular details of the activity and interactions of proteins has stemmed from the ability to isolate cDNA encoding these proteins. Computer-based analysis of deduced amino acid sequence allows
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