![HIF1 alpha antibody [GT10211]](https://img1.dxycdn.com/2022/0329/070/1976815818165020453-14.jpg)
- ￥1700 - 4000
- GeneTex
- 美国
- GTX628480
- 2025年05月19日
- WB, ICC/IF, IHC-P, IP
- Mouse
- Human, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The immunogen used to generate this antibody corresponds to human HIF1 alpha
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX628480
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
HIF1 alpha
- 抗体英文名：
HIF1 alpha antibody [GT10211]
- 抗体名：
HIF1 alpha 抗体 [GT10211]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
![HIF1 alpha antibody [GT10211]](https://img1.dxycdn.com/p/s14/2024/0412/834/6547870673006778771.jpg)
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody [GT10211] (GTX628480) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![HIF1 alpha antibody [GT10211]](https://img1.dxycdn.com/p/s14/2024/0412/706/0656034199006778771.jpg)
HIF1 alpha antibody [GT10211] detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: HIF1 alpha stained by HIF1 alpha antibody [GT10211] (GTX628480) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
![HIF1 alpha antibody [GT10211]](https://img1.dxycdn.com/p/s14/2024/0412/940/6752915394006778771.jpg)
HIF1 alpha antibody [GT10211] detects HIF1A protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract (untreated)
B. 30 μg HepG2 whole cell lysate/extract (1%O2 treatment for 48hr)
7.5% SDS-PAGE
HIF1 alpha antibody [GT10211] (GTX628480) dilution: 1:250
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![HIF1 alpha antibody [GT10211]](https://img1.dxycdn.com/p/s14/2024/0412/975/8442385094006778771.jpg)
HIF-1 alpha antibody immunoprecipitates HIF-1 alpha protein in IP experiments.
IP Sample: 1000 μg HepG2 whole cell lysate/extract
A. 40 μg HepG2 whole cell lysate/extract from normoxia
B. 40 μg HepG2 whole cell lysate/extract from hypoxia 24hr (1%O2)
C. Control with 2.5 μg of preimmune mouse IgG/HepG2 whole cell
lysate/extract from normoxia
D. Control with 2.5 μg of preimmune mouse IgG/ HepG2 whole cell
lysate/extract from hypoxia 24hr (1%O2)
E. Immunoprecipitation of HIF-1 alpha protein by 2.5 μg of HIF-1
alpha antibody (GTX628480)/HepG2 whole cell lysate/extract
from normoxia
F. Immunoprecipitation of HIF-1 alpha protein by 2.5 μg of HIF-1
alpha antibody (GTX628480)/HepG2 whole cell lysate/extract
from hypoxia 24hr (1%O2)
10% SDS-PAGE
The immunoprecipitated HIF-1 alpha protein was detected by HIF-1 alpha antibody (GTX628480) diluted at 1:500.
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
![HIF1 alpha antibody [GT10211]](https://img1.dxycdn.com/p/s14/2024/0412/736/6503399834006778771.jpg)
HIF1 alpha antibody detects HIF1 alpha protein at nuclear by confocal immunofluorescent analysis. Sample: Hypoxia (500μM CoCl2) treated 24hr (right) or untreated (left) HeLa cells were fixed in 4% PFA for 15 min. Red: HIF1 alpha protein stained by HIF1 alpha antibody (GTX628480) diluted at 1:250. Green: Alpha-tubulin, a cytoskeleton marker, stained by Rabbit Polyclonal antibody (GTX102078) diluted at 1:500. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
![HIF1 alpha antibody [GT10211]](https://img1.dxycdn.com/p/s14/2024/0412/484/2910041735006778771.jpg)
HIF1 alpha antibody [GT10211] detects HIF1 alpha protein by Western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract (untreated)
B. 30 μg HepG2 whole cell lysate/extract (500 μM CoCl2 treatment for 24 hr)
7.5 % SDS-PAGE
HIF1 alpha antibody [GT10211] (GTX628480) dilution: 1:500
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文献和实验Keitoku M et al., J Clin Med 2022 (PMID:36555975)
Yao-Lung Chang et al., Genes (Basel) 2022 (PMID:35627137)
Chen Zhang et al., Int J Med Sci 2021 (PMID:34522167)
Beak JY et al., J Biol Chem 2021 (PMID:34756888)
Sharma S et al., Hum Mol Genet 2017 (PMID:28204537)
Saki M et al., Mol Cancer Res 2017 (PMID:28801308)
Sharma S et al., Genome Biol 2019 (PMID:30791937)
Cho SY et al., Evid Based Complement Alternat Med 2013 (PMID:23589723)
Masola V et al., Oncotarget 2018 (PMID:30546836)
Shih JW et al., Nat Commun 2017 (PMID:28639619)
Matsumura-Matsuda E et al., Intern Med 2018 (PMID:29269645)
Berezowska S et al., Virchows Arch 2017 (PMID:28062918)
Bourseau-Guilmain E et al., Nat Commun 2016 (PMID:27094744)
Biao Ma et al., Nat Cell Biol. 2014 (PMID:25438054)
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