- ￥6100
- GeneTex
- 美国
- GTX60803
- 2025年07月15日
- ICC/IF, Dot, FISH, MeDIP
- Mouse
- Species independent
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
5-mC (5-methylcytosine) conjugated to BSA.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Species independent
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX60803
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
ICC/IF, Dot, FISH, MeDIP
- 浓度：
1.3 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
5-Methylcytosine / 5-mC
- 抗体英文名：
5-Methylcytosine / 5-mC antibody [GT9076]
- 抗体名：
5-Methylcytosine / 5-mC 抗体 [GT9076]
- 规格：
50 μg
ICC/IF analysis of 2.5% PFA fixed U2OS cells using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076].
Figure A : cells were immunofluorescently labelled with the 5-mC antibody after incubation of the antibody with 50 μM mCTP (left) or with DAPI (right).
Figure B : staining of the cells with the 5-mC antibody after incubation of the antibody with 50 μM hmCTP and with DAPI.
Dilution : 1:500
Dot blot analysis of 4 - 100 ng (equivalent of 5 to 0.2 pmol of C-bases) hmC, mC and C controls using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076].
Dilution : 1:600
FISH (Fluorescence in situ hybridization) analysis of metaphase chromosomes from HeLa cells (treated with 0.1 μg/ml colcemid for 1 - 2 hours) using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076].
Red : Primary antibody
Blue : DAPI
Dilution : 1:1000
ICC/IF analysis of 4% PFA-fixed mouse embryos cells fixed in the one-cellular stage (after blocking with PBS containing 2% BSA, the cells were treated with 2N HCl) using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Both the maternal (M) and paternal (P) pronuclei are stained.
Left : Primary antibody
Right : Anti- single stranded DNA antibody
Permeabilization : 0.5% Triton X-100 for 30 mins
Dilution : 1:200
ICC/IF analysis of 4% PFA fixed HeLa cells using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076].
Red : Primary antibody
Blue : DAPI
Dilution : 1:1000
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文献和实验Southern Blotting(Fred Hutchinson Cancer Research Cente
with Blot Wash #1: 2 x 5' at room temp (RT). 3. Wash with Blot Wash #2: 2 x 15' at 55℃. 4. Wash with Buffer 1: briefly (1') at RT. 5. (Optional; see above) Block with Buffer 2: 30' (O/N okay) Save and store at 4℃. 6. Incubate with antibody-conjugate
Phage‐Based Expression Cloning to Identify Interacting Proteins
the interacting protein. This technique leads directly to the isolation of a cDNA encoding the interacting protein, bypassing the need for labor?intensive protein purification, microsequencing, or antibody production.
（ min X….Mouse, Rat Sr Prot ）。 以下是圆括号中所使用的一些简称： Bov = Bovine Ck = Chicken Gt = Goat GP = Guinea Pig Sy Hms = Syrian Hamster Hrs = Horse Hu = Human Ms = Mouse Rb = Rabbit Shp
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