Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
Species independent
保质期:
12 months from the shipping date of the product.
目录编号:
GTX60803
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Mouse
应用范围:
ICC/IF, Dot, FISH, MeDIP
浓度:
1.3 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
5-Methylcytosine / 5-mC
抗体英文名:
5-Methylcytosine / 5-mC antibody [GT9076]
抗体名:
5-Methylcytosine / 5-mC 抗体 [GT9076]
规格:
50 μg
ICC/IF analysis of 2.5% PFA fixed U2OS cells using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Figure A : cells were immunofluorescently labelled with the 5-mC antibody after incubation of the antibody with 50 μM mCTP (left) or with DAPI (right). Figure B : staining of the cells with the 5-mC antibody after incubation of the antibody with 50 μM hmCTP and with DAPI. Dilution : 1:500
Dot blot analysis of 4 - 100 ng (equivalent of 5 to 0.2 pmol of C-bases) hmC, mC and C controls using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Dilution : 1:600
FISH (Fluorescence in situ hybridization) analysis of metaphase chromosomes from HeLa cells (treated with 0.1 μg/ml colcemid for 1 - 2 hours) using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Red : Primary antibody Blue : DAPI Dilution : 1:1000
ICC/IF analysis of 4% PFA-fixed mouse embryos cells fixed in the one-cellular stage (after blocking with PBS containing 2% BSA, the cells were treated with 2N HCl) using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Both the maternal (M) and paternal (P) pronuclei are stained. Left : Primary antibody Right : Anti- single stranded DNA antibody Permeabilization : 0.5% Triton X-100 for 30 mins Dilution : 1:200
ICC/IF analysis of 4% PFA fixed HeLa cells using GTX60803 5-Methylcytosine / 5-mC antibody [GT9076]. Red : Primary antibody Blue : DAPI Dilution : 1:1000