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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of human GUCY1A3 (AA: 22-214) expressed in E. Coli.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX60721
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, FACS, ELISA
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
GUCY1A3
- 抗体英文名:
GUCY1A3 antibody [3G6B2]
- 抗体名:
GUCY1A3 抗体 [3G6B2]
- 规格:
100 μg
WB analysis of HEK293 (1) and GUCY1A3 (AA: 22-214)-hIgGFc transfected HEK293 (2) cell lysate using GTX60721 GUCY1A3 antibody [3G6B2].
WB analysis of HEK293 (1) and Raji (2) cell lysate using GTX60721 GUCY1A3 antibody [3G6B2].
FACS analysis of HEK293 cells using GTX60721 GUCY1A3 antibody [3G6B2].
Green : GUCY1A3
Purple : negative control
ICC/IF analysis of HepG2 cells using GTX60721 GUCY1A3 antibody [3G6B2].
Green : GUCY1A3
Blue: DRAQ5 fluorescent DNA dye
IHC-P analysis of esophageal cancer tissue using GTX60721 GUCY1A3 antibody [3G6B2].
IHC-P analysis of renal cancer tissue using GTX60721 GUCY1A3 antibody [3G6B2].
ELISA analysis of antigen using GTX60721 GUCY1A3 antibody [3G6B2].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Protein A, derived from the cell wall of Staphylococcus aureus , and protein G, derived from the cell wall of certain strains of b -hemolytic Streptococcci , binds the Fc region
Antibody Purification: Affinity Chromatography Protein A and Protein G Sepharose
G coupled to agarose. In the Subheading 3 there are also protocols for affinity purification using a specific ligand coupled to commercial matrices like CNBr- Sepharose 4-B and Affigel.
Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L
Today, monoclonal antibodies (mAbs) form the largest category of biopharmaceuticals in clinical trials and their number is expanding rapidly (DataMonitor 2007). The antibodies or functional antibody fragments are being produced in artificial
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