- ￥5800
- GeneTex
- 美国
- GTX60399
- 2025年07月15日
- WB, IHC-P, FACS, ELISA
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant fragment of human MAP2K4 expressed in E. Coli.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60399
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, IHC-P, FACS, ELISA
- 靶点：
SEK1 / MKK4
- 抗体英文名：
SEK1 / MKK4 antibody [5H4]
- 抗体名：
SEK1 / MKK4 抗体 [5H4]
- 规格：
100 μl
WB analysis of HepG2 (1), K562 (2), and HEK293 (3) cell lysate using GTX60399 SEK1 / MKK4 antibody [5H4].
IHC-P analysis of human muscle tissue using GTX60399 SEK1 / MKK4 antibody [5H4].
FACS analysis of K562 cells using GTX60399 SEK1 / MKK4 antibody [5H4].
Green : SEK1 / MKK4
Purple : negative control
ELISA analysis of antigen using GTX60399 SEK1 / MKK4 antibody [5H4].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
of cytokine-producing cells at the single cell level. The method employs the sandwich assay approach of the enzyme-linked immunosorbent assay (ELISA), with some variations. The ELISPOT capture antibody is coated aseptically onto a polyvinylidene difluoride
Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
Intracellular Cytokine Staining Protocol
, it is common to block the directly-conjugated anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking. eBioscience antibodies are available as Functional Grade (FG) Purified (sterile
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