Histone H3 containing the monomethylated lysine 79 (H3K79me1), using a KLH-conjugated synthetic peptide.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Yeast
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX60353
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, Dot, ELISA, ChIP assay
浓度:
1.66 mg/ml (Please refer to the vial label for the specific concentration.)
ChIP analysis of HeLa cells using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence and a 1 Mb region of the X-chromosome (figure 2A and B), in 100 kb regions surrounding the GAPDH positive control and EIF4A2 genes (figure 2C and D). Antibody amount : 1μg
ELISA analysis of peptide containing the histone modification of interest using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade.
ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a region 1 kb upstream of the promoter and the coding region of the active GAPDH gene, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H3 (lane 3) using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. Dilution : 1:200
Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K79 using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. Dilution : 1:20,000