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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human ATP synthase B1. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX102980
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.45 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
ATP synthase B1
- 抗体英文名:
ATP synthase B1 antibody
- 抗体名:
ATP synthase B1 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX102980 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of methanol-fixed HeLa, using ATP synthase B1(GTX102980) antibody at 1:200 dilution.
Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
12% SDS PAGE
GTX102980 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ATP synthase B1 antibody detects ATP5F1 protein at cytosol on human colon by immunohistochemical analysis.
Sample: Paraffin-embedded colon.
ATP synthase B1 antibody (GTX102980) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验ATP或称F0F1 复合物(F0F1 complexes), 该酶在分离状态下具有ATP水解酶的活性,在结合状态下具有ATP合酶的活性, 属F型ATPase。除了线粒体中有ATP合酶外,植物叶绿体的类囊体和好氧细菌都有ATP合酶的同源物,ATP合酶的分子组成和主要特点是: 头部:头部即F1, 细菌和线粒体ATP合酶的F1都是水溶性的蛋白,结构相似,由5种多肽(α、β、γ、δ和ε)组成的九聚体(α3β3γδε),α亚基和β亚基构成一种球形的排列,头部含有三个催化ATP
Methods to Determine the Status of Mitochondrial ATP Synthase Assembly
The adenosine triphosphate (ATP) synthase (F1 -F0 complex) of the mitochondrial inner membrane is responsible for making nearly all of the ATP utilized by eukaryotic organisms. The enzyme is an oligomer of more than 20 different subunits, 14
指催化不与 ATP等核苷三磷酸分解相伴的合成反应的酶。反应形式多为与裂解相反的加成反应。在酶分类上不属于合成酶,多划在解裂酶中。柠檬酸合酶等是熟知的这种酶类。
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