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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 (Intracellular loop between domains I and II) of rat NaV1.2 (Accession : P04775).
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Rat
- 目录编号:
GTX54797
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-Fr, IP
- 浓度:
0.8 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Nav1.2
- 抗体英文名:
Nav1.2 antibody
- 抗体名:
Nav1.2 抗体
- 规格:
50 μl
IHC-Fr analysis of mouse hippocampus tissue using GTX54797 Nav1.2 antibody.
Panel A : NaV1.2 (red) is present in dendrites of pyramidal neurons in the CA3 region.
Panel B : Staining of interneurons in the pyramidal layer with mouse anti-Parvalbumin (green) demonstrates the restriction of NaV1.2 to dendrites (arrows) extending from the pyramidal layer (P).
Panel C : Confocal merge of panels A and B.
WB analysis of rat brain membrane lysate using GTX54797 Nav1.2 antibody preincubated with or without immunogen peptide.
Dilution : 1:200
IP analysis of rat brain tissue lysate using GTX54797 Nav1.2 antibody. The lower arrow indicates the IgG heavy chain.WB was performed with the GTX54797 antibody.
IP reaction : 6 μg
Lane 1 : Rat brain lysates
Lane 2 : Lysates immunoprecipitated with Anti-SCN2A (NaV1.2) Antibody
Lane 3 : Lysates immunoprecipitated with pre-immune rabbit serum
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文献和实验【操作步骤】6.2 分文章作者,亲手教你如何轻松搞定生信文章
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Cell 子刊:颜宁与西湖大学申怀宗团队合作解析 Nav1.7 高分辨率结构,有望助力止痛药研发
,Nav1.7,由 SCN9A 编码,主要在背根神经节神经元中表达,是一种很有希望的止痛药物靶点,在认知信号传导中发挥重要作用。Nav1.7 调节感觉神经元的兴奋性,并有助于几种感官模式的认知。SCN9A 的突变与原发性红热痛、与通道病相关的疼痛不敏感和阵发性极度疼痛障碍有关。 研究人员之前报道过人类 Nav1.7 的结构,在之前的研究中,一种疾病变体 Nav1.7(E406K)由于其增强了重组表达水平而被选择使用。将毒素混合后用于结构解析,最终获得了分辨率均为 3.2 Å 的低温 EM 结构
pH 0.2mol/L Na2CO3(ml) 0.2mol/L NaHCO3(ml) 9.2
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