- ￥5300
- GeneTex
- 美国
- GTX52324
- 2025年07月14日
- WB, ICC/IF, IHC-P, ELISA, IHC
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthesized peptide derived from human AhR around the phosphorylation site of Ser36
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX52324
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, ELISA, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
AHR
- 抗体英文名：
AHR (phospho Ser36) antibody
- 抗体名：
AHR (phospho Ser36) 抗体
- 规格：
100 μg
WB analysis of various samples in absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide) using GTX52324 AHR (phospho Ser36) antibody.
WB analysis of HepG2 cell lysates using GTX52324 AHR (phospho Ser36) antibody.
Left : Antibody prior incubated with antigen-specific peptide
Right : Primary antibody
ELISA analysis of phospho-peptide (P-peptide) and non-phospho-peptide(N-peptide) using GTX52324 AHR (phospho Ser36) antibody.
Peptides concentration : 1ug/ml
ICC/IF analysis of PFA-fixed HepG2 cells using GTX52324 AHR (phospho Ser36) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200 incubated with the sample for 1 hour at 37ºC
IHC-P analysis of human brain tissue sections using GTX52324 AHR (phospho Ser36) antibody.
Dilution : 1:200
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
人可溶性CD36 分子 ( sCD36 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 sCD36 单抗包被于酶标板上，标准品和样品中的 sCD36与单抗结合，加入生物素化的抗人 sCD36 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝
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