- ￥5000
- GeneTex
- 美国
- GTX32246
- 2025年07月14日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of PKC theta. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX32246
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
PKC theta (phospho Ser676)
- 抗体英文名：
PKC theta (phospho Ser676) antibody
- 抗体名：
PKC theta (phospho Ser676) 抗体
- 规格：
100 μl
WB analysis of UV-treated HeLa (A), UV-treated SP2/0 (B), UV-treated PC12 (C) whole cell lysates using GTX32246 PKC theta (phospho Ser676) antibody.
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32246 PKC theta (phospho Ser676) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
ICC/IF analysis of formalin-fixed HeLa cells using GTX32246 PKC theta (phospho Ser676) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
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文献和实验9921 Phospho-PKC Antibody Sampler Kit 9375 Phospho-PKCalpha/beta II (Thr638/641) Antibody W IP 9374 Phospho-PKCdelta (Thr505) Antibody W IHC 9376 Phospho-PKCdelta/theta (Ser643/676) Antibody W IHC 9377 Phospho-PKCtheta (Thr538
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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