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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
This antibody was produced in mice by repeated immunizations with full-length recombinant yeast SUMO protein.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Yeast
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Yeast
- 目录编号:
GTX48824
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ELISA, IHC
- 浓度:
1.9 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Sumo
- 抗体英文名:
Sumo antibody [4F2.F5.G2]
- 抗体名:
Sumo 抗体 [4F2.F5.G2]
- 规格:
50 μg
Western blot of ySUMO fusion protein. Anti-ySUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against a SUMO-GFP fusion protein (lane 2). While the actual molecular weight of the fusion protein is 39 kDa, the protein migrates as a 49 kDa band (arrowhead). No reactivity is seen for lane 1 which contains His-tagged GFP protein. The membrane was blocked using BLOTTO. Primary antibody was used at a 1:1,000 dilution in BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of IRDyeR 800 Conjugated Affinity Purified Goat-anti-Mouset IgG (H&L).
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文献和实验Studies of the Ubiquitin Proteasome System
) 6% to 8% SDS‐PAGE minigels (unit 6.1 ) Anti‐ubiquitin antibody ( protocol 31 ; for immunoblot detection) Boiling water bath Additional reagents and equipment for SDS
科研人必看:多肽、蛋白质、重组蛋白分不清? 应用场景有哪些?定制如何选?
茨海默病[4]。 场景六:多肽-药物偶联物 (PDC) 多肽-药物偶联物 (Peptide-drug conjugate, PDC) 指的是利用具有靶向能力的多肽,将小分子药物、毒素、放射性核素或功能分子定向递送至目标组织。相比传统化疗药物,PDC 通常具有更好的靶向性和相对较低的毒副作用;而相比抗体-药物偶联物 (Antibody-drug conjugate, ADC),PDC 具有分子量小、化学合成灵活易变的优势。 图 6. PDC 药物结构示意
Functional Assays on High‐Content Protein Microarrays
(SMI Radioactive) Assay on the ProtoArray Human Protein Array Basic Protocol 3: Kinase Substrate Identification (KSI) Assay on the ProtoArray Human Protein Array Basic Protocol 4: Ubiquitin/SUMO Ligase
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