- ￥1700 - 4000
- GeneTex
- 美国
- GTX102262
- 2025年07月14日
- WB, ICC/IF, IHC-P, IHC-Fr, FACS
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human p75 NGF Receptor / CD271. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX102262
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, FACS
- 浓度：
0.43 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
p75 NGF Receptor / CD271
- 抗体英文名：
p75 NGF Receptor / CD271 antibody
- 抗体名：
p75 NGF Receptor / CD271 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
p75 NGF Receptor antibody detects p75 NGF Receptor protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: p75 NGF Receptor protein stained by p75 NGF Receptor antibody (GTX102262) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Untreated (–) and treated (+) PC-3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p75 NGF Receptor / CD271 antibody (GTX102262) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
p75 NGF Receptor antibody detects p75 NGF Receptor protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p75 NGF Receptor antibody (GTX102262) at a dilution of 1:2000.
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p75 NGF Receptor / CD271 antibody (GTX102262) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
p75 NGF Receptor / CD271 antibody detects p75 NGF Receptor / CD271 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% PFA at RT for 15 min.
Green: p75 NGF Receptor / CD271 stained by p75 NGF Receptor / CD271 antibody (GTX102262) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
p75 NGF Receptor antibody detects p75 NGF Receptor protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
p75 NGF Receptor antibody (GTX102262) diluted at 1:500.
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文献和实验Zhang M et al., Front Bioeng Biotechnol 2020 (PMID:32582651)
Cell Reports：浙大陈晓团队解码肌腱成熟过程的关键细胞图谱并验证 NGF-SHP2 调控成熟的机制
形态，特征分子表达，细胞外基质超微结构和力学特性等多种指标，确定了出生后 7 天到 14 天是小鼠肌腱组织成熟的关键转变阶段。之后利用单细胞转录组技术绘制了肌腱成熟转变期的动态细胞亚群图谱及转录调控网络，发现了在发育成熟转变关键阶段富集的特异分泌神经生长因子 NGF 的 Cd9+Cd271+ 的肌腱干祖细胞亚群及受体酪氨酸激酶（Receptor tyrosine kinases）RTKs 信号通路的富集。并进一步在组织离体培养实验中验证了 NGF 的促进肌腱成熟的功能，及其下游 SHP2 活性在该功能
Cell Reports｜浙大陈晓团队解码肌腱成熟过程的关键细胞图谱
点的组织形态，特征分子表达，细胞外基质超微结构和力学特性等多种指标，确定了出生后 7 天到 14 天是小鼠肌腱组织成熟的关键转变阶段。之后利用单细胞转录组技术绘制了肌腱成熟转变期的动态细胞亚群图谱及转录调控网络，发现了在发育成熟转变关键阶段富集的特异分泌神经生长因子 NGF 的 Cd9+Cd271+ 的肌腱干祖细胞亚群及受体酪氨酸激酶（Receptor tyrosine kinases）RTKs 信号通路的富集。 并进一步在组织离体培养实验中验证了 NGF 的促进肌腱成熟的功能，及其下游 SHP
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
A. DAY 1 PROCEDURE• ABSORPTION OF THE POLYCLONAL AND PREIMMUNE SERUMThe Goat Anti-Mouse (GAM) NGF polyclonal antibody recognizes 2.5B NGF and the Goat IgG (GIG) preimmune serum serves as a control for the antibody (Hazleton). The relative signal
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