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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
HG9 cell line (small cell lung carcinoma)
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX42071
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
EpCAM
- 抗体英文名:
EpCAM antibody [VU-1D9] (Low endotoxin, azide free)
- 抗体名:
EpCAM 抗体 [VU-1D9] (Low endotoxin, azide free)
- 规格:
500 μg
FACS analysis of HT-29 cells using GTX42071 EpCAM antibody [VU-1D9] (Low endotoxin, azide free).
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文献和实验Alecia-Jane Twigger et al., Nat Commun 2022 (PMID:35091553)
Stephen Kershaw et al., BMC Cancer 2015 (PMID:25957999)
Hideharu Mikami et al., Nat Commun 2020 (PMID:32139684)
Ma R et al., Lab Invest 2014 (PMID:24776644)
Yoshida M et al., Biotechnol Bioeng 2017 (PMID:29105734)
Chen HH et al., J Biomed Opt 2014 (PMID:24723112)
Lyberopoulou A et al., PLoS One 2015 (PMID:25902072)
Hideharu Mikami et al., bioRxiv 2020 (Epub)
Purification of Endotoxin Free mAbs
pressure of circulation to force water out through the membrane, and rapidly concentrating the antibody/proteins up to 100 fold. The advantage of this method is very large volumes can be processed using sterile and endotoxin free components. Ammonium
Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
Immunocytochemistry: Common problems and some answers.
controls using other methods to demonstrate specific labeling with the antibodies being used. Change the antibody if necessary. Low amount of antigen One of the advantages of using protein A-gold to detect antibody binding
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