Store at -20ºC. Avoid multiple freeze/thaw cycles. For long-term storage -70ºC, and to prevent cross-contamination. Aliquoting is recommended.
保质期:
12 months from the shipping date of the product.
目录编号:
GTX400004
级别:
Research Kits
库存:
Available
供应商:
GeneTex
应用范围:
Dot
浓度:
50 ng/μl (Please refer to the vial label for the specific concentration.)
抗体英文名:
Methylated cytosine DNA standard kit
抗体名:
Methylated cytosine DNA standard kit
规格:
1 kit
All five standards are 426 bp with all cytosines either unmodified (lane 1, C), 5-methylcytosine (lane 2, 5mC), 5-hydroxymethylcytosine (lane 3, 5hmC), 5-formylcytosin (lane4, 5fC), 5-carboxycytosine (lane5, 5-caC). DNA marker: 1 kb Plus DNA marker.
GeneTex methylated cytosine DNA standard kit includes five recombinant double-strand DNA fragments: unmethylated cytosine DNA (C), 5-methylated cytosine DNA (5mC), 5-hydroxymethycytosine cytosine DNA (5hmC), 5-formylcytosine cytosine DNA (5fC), 5-carboxylcytosine cytosine DNA (5caC). Each methylated cytosine DNA was spotted onto a positively charged nylon membrane and subjected to Dot blot assay with corresponding antibodies. The membrane was incubated with anti-5hmc monoclonal antibody (GTX629701) at a dilution of 1:500 or with anti-5mc monoclonal antibody (GTX629448) at a dilution of 1:500