- ￥3500
- GeneTex
- 美国
- GTX400004
- 2025年07月14日
- Dot
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
Liquid
- 保存条件：
Store at -20ºC. Avoid multiple freeze/thaw cycles. For long-term storage -70ºC, and to prevent cross-contamination. Aliquoting is recommended.
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX400004
- 级别：
Research Kits
- 库存：
Available
- 供应商：
GeneTex
- 应用范围：
Dot
- 浓度：
50 ng/μl (Please refer to the vial label for the specific concentration.)
- 抗体英文名：
Methylated cytosine DNA standard kit
- 抗体名：
Methylated cytosine DNA standard kit
- 规格：
1 kit
All five standards are 426 bp with all cytosines either unmodified (lane 1, C), 5-methylcytosine (lane 2, 5mC), 5-hydroxymethylcytosine (lane 3, 5hmC), 5-formylcytosin (lane4, 5fC), 5-carboxycytosine (lane5, 5-caC). DNA marker: 1 kb Plus DNA marker.
GeneTex methylated cytosine DNA standard kit includes five recombinant double-strand DNA fragments: unmethylated cytosine DNA (C), 5-methylated cytosine DNA (5mC), 5-hydroxymethycytosine cytosine DNA (5hmC), 5-formylcytosine cytosine DNA (5fC), 5-carboxylcytosine cytosine DNA (5caC). Each methylated cytosine DNA was spotted onto a positively charged nylon membrane and subjected to Dot blot assay with corresponding antibodies.
The membrane was incubated with anti-5hmc monoclonal antibody (GTX629701) at a dilution of 1:500 or with anti-5mc monoclonal antibody (GTX629448) at a dilution of 1:500
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文献和实验Bensberg M et al., Proc Natl Acad Sci U S A 2021 (PMID:34413196)
In mammals, methylation of cytosine C-5 position is a major heritable epigenetic mark on the DNA molecule. Maintenance of proper DNA methylation patterns is a key process during embryo development and in the maintenance of adult tissue
CpG islands are clusters of CpG dinucleotides that can be found in the 5′ region of about half of human genes (1 ). Methylation of cytosine within the 5′ CpG islands is associated with transcriptional inactivation of the involved gene
The methylated DNA immunoprecipitation microarray (MeDIP-chip) is a genome-wide, high-resolution approach to detect DNA methylation in whole genome or CpG (cytosine base followed by a guanine base) islands. The method utilizes anti
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