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beta Amyloid antibody

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  • ¥5300
  • GeneTex已认证
  • 美国
  • 2025年07月12日
  • WB, ICC/IF, IHC-P, IHC-Fr, ELISA
  • Rabbit
  • Human, Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Peptide - amino acids 1-14 of human beta amyloid

    • 亚型

      IgG

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Polyclonal

    • 标记物

      Unconjugated

    • 适应物种

      Human, Mouse

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      Human

    • 目录编号

      GTX82974

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Rabbit

    • 应用范围

      WB, ICC/IF, IHC-P, IHC-Fr, ELISA

    • 浓度

      1 mg/ml (Please refer to the vial label for the specific concentration.)

    • 靶点

      beta Amyloid

    • 抗体英文名

      beta Amyloid antibody

    • 抗体名

      beta Amyloid 抗体

    • 规格

      50 μg

    产品细节图片1

    Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid (GTX82974) 1:1000 in 1% BSA-TBS-T o/n 4ºC. FEMTOMAX chemiluminescent substrate was used for detection of a 40-50 kD band consistent with a higher MW precursor form of beta amyloid. A secondary Ab only control (Shown right) showed no detectable signal.

    产品细节图片2

    Western Blot of Rabbit anti-Beta Amyloid Antibody. Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0 μg per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4ºC. Secondary antibody: Peroxidase Conjugated Goat-a-Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 40 kDa, 40 kDa for Beta Amyloid.

    产品细节图片3

    Immunohistochemistical detection of beta Amyloid using Anti-Beta Amyloid Antibody on TG APP23 mouse brain cortex frozen sections. Anti-Beta Amyloid Antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added.

    产品细节图片4

    ELISA results of GTX82974 beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1μg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated and TMB Substrate.

    产品细节图片5

    Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody (GTX82974) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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