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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide - amino acids 1-14 of human beta amyloid
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX82974
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Fr, ELISA
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
beta Amyloid
- 抗体英文名:
beta Amyloid antibody
- 抗体名:
beta Amyloid 抗体
- 规格:
50 μg
Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid (GTX82974) 1:1000 in 1% BSA-TBS-T o/n 4ºC. FEMTOMAX chemiluminescent substrate was used for detection of a 40-50 kD band consistent with a higher MW precursor form of beta amyloid. A secondary Ab only control (Shown right) showed no detectable signal.
Western Blot of Rabbit anti-Beta Amyloid Antibody. Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0 μg per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4ºC. Secondary antibody: Peroxidase Conjugated Goat-a-Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 40 kDa, 40 kDa for Beta Amyloid.
Immunohistochemistical detection of beta Amyloid using Anti-Beta Amyloid Antibody on TG APP23 mouse brain cortex frozen sections. Anti-Beta Amyloid Antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added.
ELISA results of GTX82974 beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1μg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated and TMB Substrate.
Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody (GTX82974) at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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文献和实验FA Extraction of [insoluble] Amyloid beta from Mouse Brain
plates, as a precipitate forms when samples are loaded neat. If analyzing tissue with a lot of plaques, you need larger dilution factors to analyze amyloid beta levels within the range of the standard curve. To determine amyloid beta [c] in samples
Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
) antibodies and anti-beta 2 glycoprotein I (β2-GPI) antibodies. Other autoantibodies, such as those directed against anionic phospholipids, can also be assayed; however they are not of clinical significance. Participation in a quality assurance
Evaluating Amyloid Beta (A) 1-40 Degradation by Capillary Electrophoresis
Here, we describe a capillary electrophoresis method for evaluating proteolysis of the amyloid beta peptide (Aβ) 1–40. This method is suitable for kinetic studies, demands little specialized equipment, and consumes only small
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