- ￥6300
- GeneTex
- 美国
- GTX33593
- 2025年07月11日
- WB, ICC/IF, ELISA
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX33593
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, ELISA
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
- 抗体英文名：
Histone H2B (Acetyl Lys20) antibody [RM235]
- 抗体名：
Histone H2B (Acetyl Lys20) 抗体 [RM235]
- 规格：
100 μg
ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX33593 Histone H2B (Acetyl Lys20) antibody [RM235].
Red : Primary antibody
Green : Actin
WB analysis of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate using GTX33593 Histone H2B (Acetyl Lys20) antibody [RM235].
Dilution : 0.5μg/ml
The GTX33593 reacts to Histone H2B acetylatedat Lysine 20 (K20ac). No cross reactivity with non- modified Lysine 20 or other acetylated Lysines in histone H2B.
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文献和实验组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白；在细胞正常 pH 值时，组蛋白带有正电荷，这样它们就可以和 DNA 结合，这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实，组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg ， Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白： H1 ， H2A ， H2B ， H3
Histone Acetylation/Deacetylation As a Regulator of Cell Cycle Gene Expression
-diphosphate (ADP)-ribosylation, methylation, ubiquitination, and acetylation (1 ,2 ). Histone acetylation involves the transfer of an acetyl group from acetyl-coenzyme A (CoA) to the ε-amino group of lysine side chains within the substrate by histone acetyl
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
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