- ￥5300
- GeneTex
- 美国
- GTX25432
- 2025年07月14日
- WB, ICC/IF, IP, IHC
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Intact rat mitochondria.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Rat
- 目录编号：
GTX25432
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IP, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
ATP5B
- 抗体英文名：
ATP5B antibody [4.3E8.D10]
- 抗体名：
ATP5B 抗体 [4.3E8.D10]
- 规格：
100 μg
ICC/IF analysis of HEK293T cells using GTX25432 ATP5B antibody [4.3E8.D10].
Red :ATPSynthase beta (ATP5B )
Blue : Nuclei
Fixation : formaldehyde
Permeabilization : 0.3% Triton X-100
Dilution : 1:100 overnight at 4 ºC
ICC/IF analysis of A431 cells using GTX25432 ATP5B antibody [4.3E8.D10]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
WB analysis of rat neuronal/glial cell extract using GTX25432 ATP5B antibody [4.3E8.D10].
IP analysis of THP-1 cell lysates using GTX25432 ATP5B antibody [4.3E8.D10].
GTX25432 was used as IP and IB antibody
IP reaction : 5μl antibody / 500μg lysate
Dilution : 1:1000
ICC/IF analysis of rat neuronal/glial cell culture using GTX25432 ATP5B antibody [4.3E8.D10].
ICC/IF analysis of 3T3 cells using GTX25432 ATP5B antibody [4.3E8.D10]. Cells were probed without (left) or with(right) an antibody.
Green : ATPSynthase beta (ATP5B )
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Dilution : 5 μg/ml for 1 hour at room temperature
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文献和实验Wholemount in situ hybridisation
. Each batch should ideally be tested. As a rough guide, use 5 min for E7.5, 7 min for E8.5, 9 min for E9.5, 11 min for E10.5, 12-14 min for E11.5. 8 Wash twice with PBTX for 5 minutes each. Be careful - the embryos are fragile
血清） （must be the same species as primary antibody）。This control is easy to achieve and can be used routinely in immunohistochemical staining.这个可以咨询试剂商。同型对照为免疫荧光标记中的阴性对照。由于荧光标记单抗的组来源不同，应选用相同来源的未标记单抗作为同型对照来调整背景染色。举个例子：比如检测一抗为单抗的mouse anti rat CD11b，clone OX-42 purified IgG
In Vitro Analysis of Mouse Mesencephalic Neural Crest Development
) E8.0‐8.5 mouse embryos (6 to 12 somite stages) Hanks' balanced salt solution sterilized by filtration (HBSS; see recipe
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