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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthetic peptide corresponding to residues H(394) G D R P R A S G C L A R A G(408) of mouse B1AR.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat, Cat, Primate
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX23546
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-Fr, IHC
- 靶点:
beta 1 Adrenergic Receptor
- 抗体英文名:
beta 1 Adrenergic Receptor antibody
- 抗体名:
beta 1 Adrenergic Receptor 抗体
- 规格:
100 μl
ICC/IF analysis of HeLa cells using GTX23546 beta 1 Adrenergic Receptor antibody.
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 1:100 for at least 1 hour at room temperature
IHC-Fr analysis of mouse kidney distal tubule tissue using GTX23546 beta 1 Adrenergic Receptor antibody.
WB analysis of 25 ug of various whole cell lysates using GTX23546 beta 1 Adrenergic Receptor antibody.
Dilution : 1:1000
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文献和实验Thaung HP et al., Diabetes 2015 (PMID:25784543)
Ma C et al., Onco Targets Ther 2019 (PMID:30863115)
Use of Immunohistochemistry and Confocal Microscopy in the Detection of Adrenergic Receptors
variety of these reagents, this becomes a relatively simple procedure. The procedure consists of a standard immunohistochemical protocol using the primary antibody against the adrenergic receptor followed by either fluorophore-tagged secondary antibody
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Flow Cytometry Assay to Measure Internalization of GPCRs
engineered onto the N-terminus of the GPCR.The following protocol is optimized for internalization of FLAG-tagged beta 2-adrenergic receptor (B2AR) stably transfected into HEK293 cells.Protocol 1) Grow up receptors in 37degC incubator until confluent
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