- ￥5000
- GeneTex
- 美国
- GTX18228
- 2025年07月15日
- ICC/IF, FACS
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性：
Monoclonal
- 标记物：
PE
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX18228
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
ICC/IF, FACS
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody reacts with Class III epitope on CD34 (Mucosialin). The antibody 4H11[APG] completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
- 抗体英文名：
CD34 antibody [4H11(APG)] (PE)
- 抗体名：
CD34 抗体 [4H11(APG)] (PE)
- 规格：
100 test
ICC/IF analysis of MOLM-7 cells using GTX18228 CD34 antibody [4H11(APG)] (PE).
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文献和实验Tsu-Kung Lin et al., Oxidative Medicine and Cellular Longevity 2019 2019()
Lin TK et al., Oxid Med Cell Longev 2019 (PMID:31249652)
Western Blotting with Horseradish Pe
7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED IN ALL STEPS
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
与在各种检测方案中所应用的不同技术。并就外周血干细胞计数标准方案达成了一致。推荐方案中要求标本需预先作白细胞计数，用来染色的标本体积根据对 CD34+ 细胞数量估算来决定，分析时要求收集足够的有统计学意义的标本数量。方案中推荐使用双标记方法染色，其中包括将 2 ul 的 PE 标记的 CD3 抗体和 10ul 的 FITC 标记的 CD34 抗体（ QBEnd10 ）加入 250ul 的全血进行染色，室温下孵育 20 分钟，每 5 分钟用移液器吹打混匀一次；随后加入 Ortho 公司的红细胞裂解液孵育
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