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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Extracellular domain of mouse IFNAR-1
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX14637
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, Neutralizing/Inhibition
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
IFNAR1
- 抗体英文名:
IFNAR1 antibody [MAR1-5A3]
- 抗体名:
IFNAR1 抗体 [MAR1-5A3]
- 规格:
250 μg
WB analysis of purified IFNAR1 recombinant protein using GTX14637 IFNAR1 antibody [MAR1-5A3].
Lane 1 : 0.5 ug reduced purified mouse IFNAR-1 with histidine tag at C-terminus
Lane 2 : 0.25 ug reduced purified mouse IFNAR-1 with histidine tag at C-terminus
Lane 3 : 0.125 ug reduced purified mouse IFNAR-1 with histidine tag at C-terminus.
Dilution : 8 ug/ml
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文献和实验Lee MH et al., J Leukoc Biol 2019 (PMID:31216373)
Probing and Stripping of Western Blot
of the antibodies.3. Rinse the membrane with TBSTM and place the membrane with protein side down in a minimal volume of TBSTM (3-5 ml in a square petri dish works well).4. Add antisera or antibody at the desired dilution (typically 100-5000 fold dilution dependent
Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
Intracellular Cytokine Staining Protocol
, it is common to block the directly-conjugated anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking. eBioscience antibodies are available as Functional Grade (FG) Purified (sterile
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