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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
This antibody was generated by immunizing mice with a synthetic peptide of human Presenilin-2 (PS-2).
- 亚型:
IgM
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX13926
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB
- 靶点:
Presenilin 2
- 抗体英文名:
Presenilin 2 antibody [198C679.2.1]
- 抗体名:
Presenilin 2 抗体 [198C679.2.1]
- 规格:
200 μl
WB analysis of HL-60 cell lysate using GTX13926 Presenilin 2 antibody [198C679.2.1].
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文献和实验to 1 liter with Qcheck to make sure pH is 3.0(2) Affigel Blue Running Buffer10 mM K2HPO4 2.28 g K2HPO40.15 M NaCl 8.2 g NaCl0.02% azide 0.2 g Na azideup to 1 liter with Q(3) 1.4 M NaCl8.1 g NaClup to 100 ml with Q(4) Saturated NH4SO4767 g NH4SO4add 1 liter Q
(either undiluted or 1:1 with PBT) or diluted polyclonal antiserum and incubate at 4 C overnight 8. Rinse with PBT 3 times, then wash 4 times for 15 minutes each 9. Add secondary antibody (usually 1:200 in PBT) and incubate 2 hours at room temperature
with 6 ml Buffer B, 6 ml Buffer A, and 6 ml Buffer B. Wash with Storage Buffer and hold at 4°C. • To purify the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes. • Wash with 50 ml 10 mM Tris
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