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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Enterovirus 71 VP1 (EV71 virus (strain USA/BrCr/1970)). The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Enterovirus 71
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Enterovirus 71
- 目录编号:
GTX132338
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody was raised against Enterovirus 71 VP1, and it does not cross-react with Enterovirus D68 VP1.
- 抗体英文名:
Enterovirus 71 VP1 antibody
- 抗体名:
Enterovirus 71 VP1 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132338) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132338) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
EV71 VP1 antibody detects EV71 VP1 protein by immunofluorescent analysis. Sample: RD cells infected with EV71 were fixed in 4% PFA. Green: EV71 VP1 protein stained by EV71 VP1 antibody (GTX132338) diluted at 1:100. Blue: DAPI staining. Scale bar = 500 μm.
EV71 VP1 antibody detects EV71 VP1 protein in EV71 infected mouse skeletal muscle by immunohistochemical analysis. Sample: Paraffin-embedded EV71 infected mouse skeletal muscle. EV71 VP1 antibody [GTX132338] diluted at 1:500
Antigen Retrieval: Citrate buffer
Non-infected (–) and infected RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus 71 VP1 antibody (GTX132338) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- 作者
- 内容
- 询问日期
文献和实验Han Kang Tee et al., bioRxiv 2019 (Epub)
Chen D et al., Int Immunopharmacol 2018 (PMID:29730555)
Fu Y et al., PLoS Pathog 2023 (PMID:36634130)
Cui G et al., Front Pharmacol 2022 (PMID:36386168)
Hsieh CF et al., J Virol 2022 (PMID:35319229)
Xu M et al., PLoS Pathog 2022 (PMID:36070294)
Yuanmin Dong et al., Front Microbiol 2021 (PMID:35185830)
Han Fu et al., Am J Transl Res 2020 (PMID:33437396)
Zhao X et al., J Virol 2022 (PMID:35170979)
Chen D et al., Front Immunol 2018 (PMID:29922292)
Du M et al., Anal Chem 2019 (PMID:31698906)
Guo H et al., Nat Commun 2019 (PMID:31492846)
Wang H et al., Int J Mol Sci 2019 (PMID:31575039)
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction
?protein interaction is described using an antibody and its antigen as an example. The affinity of the antibody for its antigen is determined by measuring the binding kinetics of the interaction. The protocols are divided into three major steps
【求助】我在genebank提交核苷酸序列遇到困难,请求帮助
enterovirus 71 strain NBChina01 from China,complete cds. ACCESSION VERSION KEYWORDS . SOURCE Human enterovirus 71 ORGANISM Human enterovirus 71 Viruses; ssRNA positive-strand viruses, no DNA stage; Picornavirales; Picornaviridae
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