- ￥5300
- GeneTex
- 美国
- GTX79055
- 2025年07月14日
- WB, IHC-P
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against synthesized phosphopeptide derived from human Histone H3.1 around the phosphorylation site of Threonine 3 (A-R-Tp-K-Q).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX79055
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H3T3ph (phospho Thr3)
- 抗体英文名：
Histone H3T3ph (phospho Thr3) antibody
- 抗体名：
Histone H3T3ph (phospho Thr3) 抗体
- 规格：
100 μg
WB analysis of HUVEC cells treated with serum lysate using GTX79055 Histone H3T3ph (phospho Thr3) antibody.
Left : Primary antibody
Right : Primary antibody preincubated with immunogen peptide
IHC-P analysis of human malignant lymphoma tissue using GTX79055 Histone H3T3ph (phospho Thr3) antibody.
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
The optimal exposure time will depend on the amount of 32 P used and the amount of kinase activity. Generally a few hours should be fine. SOLUTIONS Histone Wash Buffer 25 mM Tris HCl, pH7.5 70 mM NaCl 10 mM MgCl2 1 mM DTT
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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