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- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the N-terminus region of human TRRAP. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX129505
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IP, ChIP assay
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
TRRAP
- 抗体英文名:
TRRAP antibody
- 抗体名:
TRRAP 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
TRRAP antibody detects TRRAP protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
5 % SDS-PAGE
TRRAP antibody (GTX129505) dilution: 1:500
TRRAP antibody detects TRRAP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5 % SDS-PAGE
TRRAP antibody (GTX129505) dilution: 1:500
TRRAP antibody detects TRRAP protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: TRRAP protein stained by TRRAP antibody (GTX129505) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-TRRAP antibody. The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.
TRRAP antibody immunoprecipitates TRRAP protein in IP experiments.
IP samples: 293T whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of TRRAP protein by 4 μg TRRAP antibody (GTX129505)
C : Immunoprecipitation of TRRAP by 4 μg of TRRAP antibody (GTX129507)
5 % SDS-PAGE
The immunoprecipitated TRRAP protein was detected by TRRAP antibody (GTX129505) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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