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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 beta. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX128795
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
HIF1 beta
- 抗体英文名:
HIF1 beta antibody
- 抗体名:
HIF1 beta 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
HIF1 beta antibody detects HIF1 beta protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
HIF1 beta antibody (GTX128795) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
HIF1 beta antibody detects HIF1 beta protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract (untreated)
B. 30 μg HepG2 whole cell lysate/extract ( 1% O2 treatment for 24 hr)
7.5 % SDS-PAGE
HIF1 beta antibody (GTX128795) dilution: 1:1000
HIF1 beta antibody detects HIF1 beta protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
7.5 % SDS-PAGE
HIF1 beta antibody (GTX128795) dilution: 1:1000
HIF1 beta antibody detects HIF1 beta protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 500μM CoCl2 for 24hr (right) were fixed in 4% PFA at RT for 15 min.
Green: HIF1 beta protein stained by HIF1 beta antibody (GTX128795) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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in 200 mls Solution A. Repeat wash. Pellet cells as above and resuspend in 4 mls Solution A. Reserve 2 mls of the cell suspension. Spheroplast the remaining 2 ml of the cell suspension by addition of beta-mercaptoethanol to 0.1, glusulase
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