Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of mCherry. The exact sequence is proprietary.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
保质期:
12 months from the shipping date of the product.
目录编号:
GTX128509
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, IP
浓度:
0.88 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
mCherry
抗体英文名:
mCherry antibody
抗体名:
mCherry 抗体
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
mcherry antibody immunoprecipitates mcherry protein in IP experiments.IP Sample: 293T whole cell lysate/extractA : 20 μg whole cell lysate/extract of mcherry protein expressing 293T cellsB : Control with 2.5 μg of pre-immune rabbit IgGC : Immunoprecipitation of mcherry protein by 2.5 μg of mcherry antibody (GTX128509) 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody (GTX128509) diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128509) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Application Reference
Periasamy J et al., Cell Chem Biol 2018 (PMID:29606576)
Cristini A et al., Cell Rep 2019 (PMID:31533039)
Davidson et al., Current Biology 2019 (Epub)
Wang W et al., Dev Comp Immunol 2019 (PMID:30414403)
Davidson PM et al., EMBO Rep 2020 (PMID:32419336)