- ￥1700 - 4000
- GeneTex
- 美国
- GTX128091
- 2025年07月04日
- ICC/IF, IHC-P, IHC-Fr, FACS, IHC
- Rabbit
- Species independent
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The immunogen used to generate this antibody corresponds to BrdU
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Species independent
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX128091
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
ICC/IF, IHC-P, IHC-Fr, FACS, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
BrdU
- 抗体英文名：
BrdU antibody
- 抗体名：
BrdU 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
BrdU antibody detects Bromodeoxyuridine (BrdU) in mouse BrdU treated intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestinal tissue pulsed with BrdU.
BrdU stained by BrdU antibody (GTX128091) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BrdU antibody detects BrdU protein at nucleus and mitochondria by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: BrdU stained by BrdU antibody (GTX128091) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).
BrdU antibody (GTX128091) detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% PFA at 4oC for 15 minutes by flow cytometry analysis.
Black: Unlabelled sample was used as a control.
Red: BrdU antibody (GTX128091) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
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文献和实验Ni YL et al., Chem Biol Interact 2018 (PMID:29908167)
Zhu B et al., Cell Mol Life Sci 2018 (PMID:29302702)
Sun J et al., Brain Res 2013 (PMID:23148948)
Stegeman H et al., Radiother Oncol 2013 (PMID:23453541)
Chen SY et al., Mol Ther Methods Clin Dev 2022 (PMID:35846572)
Lanzafame M et al., Nucleic Acids Res 2021 (PMID:34581821)
Hattori Y et al., Biochem Pharmacol 2021 (PMID:33711284)
H?rdtner C et al., Basic Res Cardiol 2020 (PMID:33296022)
Lazarchuk P et al., Mol Cell Biol 2020 (PMID:32015101)
Liu G et al., Int J Biol Sci 2019 (PMID:31337969)
Guosheng Liu et al., Int J Biol Sci 2019;15(7)
Li HY et al., J Agric Food Chem 2019 (PMID:30661353)
a variety of cellular sources from human olfactory epithelium (Hahn et al., 2005) and neural progenitors (Nieoullon et al., 2005) to neural stem cells (Chu et al., 2004). BrdU antibody from Abcam Identification of the labelled cells can be applied
实验材料 Materials Required But Not Provided 1. BrdU reagent (Invitrogen Cat. No. 00-0103) 2. Cytospin Tubes 3. Phosphate-Buffered Saline 4. 0.22 micron filter 实验步骤 1. Labeling with BrdU In Vivo
Anti-BrdU-FITC (Becton Dickinson or Phoenix Flow) 0.15 M NaCl, 1.5 M NaCl 10 Paraformaldehype (kept as stock in -80°C) Tween 1M MgCl2 FACS Tubes Protocol: Cells in 96 well FACS plate Block with 24G-2 Surface stain cells
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