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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys5/Lys8/Lys12/Lys16 of human Histone H4. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX122653
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, Dot
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16)
- 抗体英文名:
Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody
- 抗体名:
Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Dotblot analysis of anti-Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody with peptide samples.
Peptide samples (0.1 μg) were spotted onto positively charged nylon membrane and blotted with Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody (GTX122653) at different dilution as indicated.
A: Peptide samples of Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16)
B: Peptide samples of Histone H4
Sample (30 ug of whole cell lysate)
A: PC-12
15% SDS PAGE
GTX122653 diluted at 1:10000
Sample (30 ug of whole cell lysate)
A: HeLa
15% SDS PAGE
GTX122653 diluted at 1:10000
Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody detects Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein stained by Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody (GTX122653) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: BCL-1
15% SDS PAGE
GTX122653 diluted at 1:10000
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16)(GTX122653) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Hasebe T et al., Gen Comp Endocrinol 2020 (PMID:32084349)
Tsai CH et al., Biochim Biophys Acta Mol Cell Res 2019 (PMID:31672612)
Bai B et al., Birth Defects Res 2018 (PMID:30114346)
Liu MK et al., Int J Mol Sci 2016 (PMID:27314325)
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