- ￥1700 - 4000
- GeneTex
- 美国
- GTX122648
- 2025年07月16日
- WB, ICC/IF, Dot
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys9/Lys14 of human Histone H3. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX122648
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, Dot
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H3K9K14ac (Acetyl Lys9/Lys14)
- 抗体英文名：
Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody
- 抗体名：
Histone H3K9K14ac (Acetyl Lys9/Lys14) 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Dotblot analysis of anti-Histone H3 (acetyl Lys 9, Lys14) antibody with peptide samples.
Peptide samples (0.1 μg) were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys 9, Lys14) antibody (GTX122648) at different dilution as indicated.
A: Peptide samples of Histone H3.1
B: Peptide samples of Histone H3 (acetyl Lys 9, Lys14)
Histone H3 (acetyl Lys 9, Lys14) antibody detects Histone H3 (acetyl Lys 9, Lys14) protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Trichostatin treatment for 18 hr)
15% SDS-PAGE
Histone H3 (acetyl Lys 9, Lys14) antibody (GTX122648) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H3K9K14ac (acetyl Lys9/Lys14) antibody (GTX122648) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H3K9K14ac (acetyl Lys9/Lys14) protein stained by Histone H3K9K14ac (acetyl Lys9/Lys14) antibody (GTX122648) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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文献和实验Chang TM et al., Cancer Sci 2020 (PMID:33128283)
Chang MC et al., Int J Mol Sci 2018 (PMID:30562925)
Hou MF et al., Exp Cell Res 2017 (PMID:28842166)
Yu-Ting Peng et al., Toxicology Reports 2015 2()
Lee KH et al., Sci Rep 2014 (PMID:25227736)
Wang C et al., Int J Mol Sci 2023 (PMID:36902234)
Simona Dedoni et al., Int J Mol Sci 2022 (PMID:35409209)
Simona Dedoni et al., Int J Mol Sci 2021 (PMID:34360553)
Histone Deacetylase Activity Assay
Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from the ɛ-amino groups of conserved lysine residues in the amino terminal tail of histones. In humans, there are 18 potential deacetylase enzymes
-mediated pathways in the nucleus, Nature Reviews Genetics, 6(1): 24-35. 3.Morris, K.V. (2005) siRNA-mediated transcriptional gene silencing: the potential mechanism and a possible role in the histone code, Cell and Molecular Life Sciences, 62(24): 3057
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
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